BerkiGEM2007-ArthurConstructionFile13

From 2007.igem.org

(Difference between revisions)
 
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digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest
 +
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy
 +
</pre>
 +
 +
Another version tried...
 +
 +
<pre>
 +
construct ptet/rbs/wbbL/rbs/neuS in lo copy
 +
 +
digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
 +
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, BglI) 2146+1149+914, largest
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy
</pre>
</pre>

Latest revision as of 23:28, 3 July 2007

construct ptet/rbs/wbbL/rbs/neuS in lo copy

digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy

Another version tried...

construct ptet/rbs/wbbL/rbs/neuS in lo copy

digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, BglI) 2146+1149+914, largest
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy