From 2007.igem.org

Revision as of 23:30, 3 July 2007 (view source) Ayu (Talk | contribs) ← Older edit		Latest revision as of 18:34, 10 July 2007 (view source) Ayu (Talk | contribs)
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	Template for all: I716004		Template for all: I716004
		+	For each, digest PCR prod with BglII/XhoI and ligate into pBca9145-1145 #4 (BglII/XhoI)
-	1) ay008/9      349bp	+	(I716013) (yfbE/rbs/ATG)    ay008/9      349bp
-	2) ay008/10    333bp	+	(I716014) (yfbE solo)      ay008/10    333bp
-	3) ay011/12    709bp	+	(I716015) (RFP without ATG) ay011/12    709bp
	</pre>		</pre>

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Latest revision as of 18:34, 10 July 2007

8   Forward primer.   
cgacgAGATCTgctaacacgtaaactccac

9   Reverse to get the RBS, and an ATG (what they did)
gcgtgCTCGAGttaGGATCCcattgcttttcctt

10  Reverse to get without an RBS (unsure if this will work)
gcataCTCGAGttaGGATCCgccattgaatacctgtcca

13  Forward get RFP w/o start codon
cgacgAGATCTgcttcctccgaagacgttatc

14  Reverse get RFP
gcataCTCGAGttaGGATCCttattaagcaccggtggag



pcr 7/3

Template for all: I716004
For each, digest PCR prod with BglII/XhoI and ligate into pBca9145-1145 #4 (BglII/XhoI)


(I716013) (yfbE/rbs/ATG)    ay008/9      349bp
(I716014) (yfbE solo)       ay008/10     333bp
(I716015) (RFP without ATG) ay011/12     709bp