Revision as of 22:22, 3 August 2007 by Ayu (Talk | contribs)
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making basic biobrick I716028 and 29 - Mg2+ repressed promoter (p-mgrB)

[pcr] AY021/22 21/23 onto MG1655 genome   (550 bp, BglII/XhoI)
put it into pBca9145
you get I716028 and I716029

mgrB attempt by yu (550 bp)
AY021 (f)  catggAGATCTtgagagtaagaacctgtcgg 
AY022 (r1) gtacaCTCGAGttaGGATCCgtttcacctaccttatgtc
AY023 (r2) gtacaCTCGAGttaGGATCCcgaaactttttcactccaatc