From 2007.igem.org

making basic biobrick I716028 and 29 - Mg2+ repressed promoter (p-mgrB)

[pcr] AY021/22 21/23 onto MG1655 genome   (550 bp, BglII/XhoI)
put it into pBca9145
you get I716028 and I716029


mgrB attempt by yu (550 bp)
AY021 (f)  catggAGATCTtgagagtaagaacctgtcgg 
AY022 (r1) gtacaCTCGAGttaGGATCCgtttcacctaccttatgtc
AY023 (r2) gtacaCTCGAGttaGGATCCcgaaactttttcactccaatc