PCR ca1099F/AD0005R on 9145-I716062 -HbA alpha 2X mutant- (1445 bp) PCR AD0006F/ca1099R on 9145-I716062 –HbA alpha 2X mutant- (1577 bp) Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716068