BerkiGEM2007-AustinConstructionFile20
From 2007.igem.org
(Difference between revisions)
Line 6: | Line 6: | ||
Product is ADtemp3<br> | Product is ADtemp3<br> | ||
_______________________________________________________<br> | _______________________________________________________<br> | ||
- | + | ||
- | + | ||
- | Pcr CA1154F/CA1099R on | + | Pcr CA1154F/CA1099R on I716070 (1607 +TT bp)<br> |
Product is ADtemp5<br> | Product is ADtemp5<br> | ||
____________________________________________________________<br> | ____________________________________________________________<br> | ||
Digest ADtemp3 (BsaI/BamHI) <br> | Digest ADtemp3 (BsaI/BamHI) <br> | ||
Digest ADtemp5 (BsaI/BglII)<br> | Digest ADtemp5 (BsaI/BglII)<br> | ||
- | Ligate, product is: | + | Ligate, product is: I716071 |
<br> | <br> |
Revision as of 18:01, 25 June 2007
pcr CA1150F/G00101 on 9145-I716054 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
_______________________________________________________
Pcr CA1154F/CA1099R on I716070 (1607 +TT bp)
Product is ADtemp5
____________________________________________________________
Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716071