BerkiGEM2007-AustinConstructionFile20

(Difference between revisions)

Latest revision as of 18:01, 25 June 2007

Digest 9145-bca1092 (Bgl2/EcoR1, 2206/3, Larger)
Digest I716053 (BamH1/EcoR1, 2063/444, Smaller)
Ligated product is I716070

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-pcr CA1150F/G00101 on 9145-I716054 (575 bp)
+Digest 9145-bca1092 (Bgl2/EcoR1, 2206/3, Larger)<br>
-Product is ADtemp1
+Digest I716053 (BamH1/EcoR1, 2063/444, Smaller)<br>
-Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)
+Ligated product is I716070<br>
-Product is ADtemp2
-pcr CA1099F/D58 on ADtemp2 (3465 bp)
-Product is ADtemp3
-_______________________________________________________
-Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below.
-Call the alpha-TT construct ADtemp4
-Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp)
-Product is ADtemp5
-____________________________________________________________
-Digest ADtemp3 (BsaI/BamHI)
-Digest ADtemp5 (BsaI/BglII)
-Ligate, product is: I716070