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BerkiGEM2007-AustinConstructionFile20 - Revision history
2024-03-28T17:07:22Z
Revision history for this page on the wiki
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http://2007.igem.org/wiki/index.php?title=BerkiGEM2007-AustinConstructionFile20&diff=4495&oldid=prev
AustinDay at 18:01, 25 June 2007
2007-06-25T18:01:46Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:01, 25 June 2007</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">pcr CA1150F/G00101 on </del>9145-<del class="diffchange diffchange-inline">I716054 (575 bp)<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Digest </ins>9145-<ins class="diffchange diffchange-inline">bca1092 </ins>(Bgl2/<ins class="diffchange diffchange-inline">EcoR1, 2206</ins>/<ins class="diffchange diffchange-inline">3, Larger</ins>)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Product is ADtemp1<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Digest <ins class="diffchange diffchange-inline">I716053 </ins>(<ins class="diffchange diffchange-inline">BamH1</ins>/<ins class="diffchange diffchange-inline">EcoR1, 2063</ins>/<ins class="diffchange diffchange-inline">444, Smaller</ins>)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Sub ADtemp1 into pTRC plasmid </del>(Bgl2/<del class="diffchange diffchange-inline">XhoI)<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Ligated </ins>product is <ins class="diffchange diffchange-inline">I716070</ins><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Product is ADtemp2<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">pcr CA1099F</del>/<del class="diffchange diffchange-inline">D58 on ADtemp2 (3465 bp</del>)<del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Product is ADtemp3<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">_______________________________________________________<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Pcr CA1154F/CA1099R on I716070 (1607 +TT bp)<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Product is ADtemp5<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">____________________________________________________________</del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Digest <del class="diffchange diffchange-inline">ADtemp3 </del>(<del class="diffchange diffchange-inline">BsaI</del>/<del class="diffchange diffchange-inline">BamHI) <br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Digest ADtemp5 (BsaI</del>/<del class="diffchange diffchange-inline">BglII</del>)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Ligate, </del>product is<del class="diffchange diffchange-inline">: I716071</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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AustinDay
http://2007.igem.org/wiki/index.php?title=BerkiGEM2007-AustinConstructionFile20&diff=4494&oldid=prev
AustinDay at 18:01, 25 June 2007
2007-06-25T18:01:24Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:01, 25 June 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Product is ADtemp3<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Product is ADtemp3<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>_______________________________________________________<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>_______________________________________________________<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below. <br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Call the alpha-TT construct ADtemp4<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Pcr CA1154F/CA1099R on <del class="diffchange diffchange-inline">ADtemp4 </del>(1607 +TT bp)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Pcr CA1154F/CA1099R on <ins class="diffchange diffchange-inline">I716070 </ins>(1607 +TT bp)<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Product is ADtemp5<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Product is ADtemp5<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>____________________________________________________________<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>____________________________________________________________<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digest ADtemp3 (BsaI/BamHI) <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digest ADtemp3 (BsaI/BamHI) <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digest ADtemp5 (BsaI/BglII)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digest ADtemp5 (BsaI/BglII)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Ligate, product is: <del class="diffchange diffchange-inline">I716070</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Ligate, product is: <ins class="diffchange diffchange-inline">I716071</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
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AustinDay
http://2007.igem.org/wiki/index.php?title=BerkiGEM2007-AustinConstructionFile20&diff=4445&oldid=prev
AustinDay at 08:09, 25 June 2007
2007-06-25T08:09:18Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:09, 25 June 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>pcr CA1150F/G00101 on 9145-I716054 (575 bp)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>pcr CA1150F/G00101 on 9145-I716054 (575 bp)<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Product is ADtemp1</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Product is ADtemp1<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Product is ADtemp2</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Product is ADtemp2<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>pcr CA1099F/D58 on ADtemp2 (3465 bp)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>pcr CA1099F/D58 on ADtemp2 (3465 bp)<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Product is ADtemp3</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Product is ADtemp3<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>_______________________________________________________</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>_______________________________________________________<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below. <ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Call the alpha-TT construct ADtemp4</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Call the alpha-TT construct ADtemp4<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp)<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Product is ADtemp5</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Product is ADtemp5<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>____________________________________________________________</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>____________________________________________________________<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Digest ADtemp3 (BsaI/BamHI) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Digest ADtemp3 (BsaI/BamHI) <ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Digest ADtemp5 (BsaI/BglII)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Digest ADtemp5 (BsaI/BglII)<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligate, product is: I716070</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligate, product is: I716070</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
</table>
AustinDay
http://2007.igem.org/wiki/index.php?title=BerkiGEM2007-AustinConstructionFile20&diff=4444&oldid=prev
AustinDay at 08:08, 25 June 2007
2007-06-25T08:08:48Z
<p></p>
<p><b>New page</b></p><div>pcr CA1150F/G00101 on 9145-I716054 (575 bp)<br />
Product is ADtemp1<br />
Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)<br />
Product is ADtemp2<br />
pcr CA1099F/D58 on ADtemp2 (3465 bp)<br />
Product is ADtemp3<br />
_______________________________________________________<br />
Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below. <br />
Call the alpha-TT construct ADtemp4<br />
Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp)<br />
Product is ADtemp5<br />
____________________________________________________________<br />
Digest ADtemp3 (BsaI/BamHI) <br />
Digest ADtemp5 (BsaI/BglII)<br />
Ligate, product is: I716070</div>
AustinDay