BerkiGEM2007-AustinConstructionFile20

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pcr CA1150F/G00101 on 9145-I716054 (575 bp) Product is ADtemp1 Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI) Product is ADtemp2 pcr CA1099F/D58 on ADtemp2 (3465 bp) Product is ADtemp3 _______________________________________________________ Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below. Call the alpha-TT construct ADtemp4 Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp) Product is ADtemp5 ____________________________________________________________ Digest ADtemp3 (BsaI/BamHI) Digest ADtemp5 (BsaI/BglII) Ligate, product is: I716070

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