From 2007.igem.org

pcr CA1150F/G00101 on 9145-I716054 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
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Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below.
Call the alpha-TT construct ADtemp4
Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp)
Product is ADtemp5
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Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716070