BerkiGEM2007-AustinConstructionFile26

From 2007.igem.org

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Alpha: <br>
Alpha: <br>
-
PCR CA1150/CA1099R on I716078 (1727 Bp)<br>
+
PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)<br>
-
Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br>
+
Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.<br> <br>
Beta: <br>
Beta: <br>
-
PCR CA1099R/D58 on Part Ptrc-I716081 <br>
+
PCR CA1099F/G00101 on Part 9145-I716081 <br>
-
Digest (Bsa1/BamH1) and ligate with alpha portion. <br>
+
Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion. <br>
<br><br>
<br><br>
-
Product is pTRC-I716076 (Second best all around)
+
Product is 9145-I716076 (Second best all around)

Latest revision as of 18:31, 5 July 2007

Alpha:
PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)
Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.

Beta:

PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion.


Product is 9145-I716076 (Second best all around)