BerkiGEM2007-AustinConstructionFile53

From 2007.igem.org

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PCR AD0012F/AD0012R on K-12 strain genome (2701 bp)<br>
PCR AD0012F/AD0012R on K-12 strain genome (2701 bp)<br>
-
Post PCR digest with (NdeI/BglII/DpnI) <br>
+
Post PCR digest with (NdeI/XhoI/DpnI) <br>
-
Digest petDUET (NdeI/BglII, 5412/8, Larger)<br>  
+
Digest petDUET (NdeI/XhoI, 5412/8, Larger)<br>  
Ligation product is I716353<br>
Ligation product is I716353<br>

Latest revision as of 01:28, 11 August 2007

PCR AD0012F/AD0012R on K-12 strain genome (2701 bp)
Post PCR digest with (NdeI/XhoI/DpnI)

Digest petDUET (NdeI/XhoI, 5412/8, Larger)

Ligation product is I716353