BerkiGEM2007-KristinConstructionFile1
From 2007.igem.org
(Difference between revisions)
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PCR kd003/kd002 on hemA (618 bp, gp=B) | PCR kd003/kd002 on hemA (618 bp, gp=B) | ||
-------------------------------------------------------- | -------------------------------------------------------- | ||
| - | PCR kd001/kd002 on A+B | + | PCR kd001/kd002 on A+B (1237 bp, Bg1II/XhoI) |
Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) | Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) | ||
Product is pBca9145-I716151 | Product is pBca9145-I716151 | ||
-------------------------------------------------------- | -------------------------------------------------------- | ||
| - | kd001 Forward Bg1II for hemA | + | kd001 Forward Bg1II for hemA ccaaaAGATCTatggactacaatctcgcgctc |
| - | kd002 Reverse Xho1 for hemA | + | kd002 Reverse Xho1 for hemA gctagCTCGAGttaGGATCCtcacgcacagcgcgcccagag |
kd003 Removing the EcoR1 site in hemA cgccgatGAGTTCggcgcgc | kd003 Removing the EcoR1 site in hemA cgccgatGAGTTCggcgcgc | ||
kd004 Removing the EcoR1 site in hemA gcgcgccGAACTCatcggcg | kd004 Removing the EcoR1 site in hemA gcgcgccGAACTCatcggcg | ||
</pre> | </pre> | ||
Latest revision as of 21:09, 6 June 2007
Construction of hemA basic part from R. capsulatus PCR kd001/kd004 on hemA (639 bp, gp=A) PCR kd003/kd002 on hemA (618 bp, gp=B) -------------------------------------------------------- PCR kd001/kd002 on A+B (1237 bp, Bg1II/XhoI) Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) Product is pBca9145-I716151 -------------------------------------------------------- kd001 Forward Bg1II for hemA ccaaaAGATCTatggactacaatctcgcgctc kd002 Reverse Xho1 for hemA gctagCTCGAGttaGGATCCtcacgcacagcgcgcccagag kd003 Removing the EcoR1 site in hemA cgccgatGAGTTCggcgcgc kd004 Removing the EcoR1 site in hemA gcgcgccGAACTCatcggcg
