BerkiGEM2007-KristinConstructionFile3
From 2007.igem.org
(Difference between revisions)
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Construction of hemC basic part | Construction of hemC basic part | ||
PCR kd007/kd0010 on hemC (306 bp, gp=A) | PCR kd007/kd0010 on hemC (306 bp, gp=A) | ||
| - | PCR kd009/kd008 on hemC (688 bp, gp=B) | + | PCR kd009/kd008 on hemC (688 bp, gp=B) |
-------------------------------------------------------- | -------------------------------------------------------- | ||
| - | PCR kd007/kd008 on A+B | + | PCR kd007/kd008 on A+B (994 bp, Bg1II/XhoI) |
Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) | Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) | ||
Product is pBca9145-I716154 | Product is pBca9145-I716154 | ||
| Line 10: | Line 10: | ||
kd007 Forward Bg1II for hemC ccaaaAGATCTatgataatgacggtaacaagc | kd007 Forward Bg1II for hemC ccaaaAGATCTatgataatgacggtaacaagc | ||
kd008 Reverse Xho1 for hemC gctagCTCGAGttaGGATCCtcatgccggggcgtctccgttatag | kd008 Reverse Xho1 for hemC gctagCTCGAGttaGGATCCtcatgccggggcgtctccgttatag | ||
| - | kd009 | + | kd009 Forward remove the EcoR1 site in hemC gccggttGAGTTCccgcaag |
kd010 Reverse remove the EcoR1 site in hemC cttgcggGAACTCaaccggc | kd010 Reverse remove the EcoR1 site in hemC cttgcggGAACTCaaccggc | ||
</pre> | </pre> | ||
Latest revision as of 21:11, 6 June 2007
Construction of hemC basic part PCR kd007/kd0010 on hemC (306 bp, gp=A) PCR kd009/kd008 on hemC (688 bp, gp=B) -------------------------------------------------------- PCR kd007/kd008 on A+B (994 bp, Bg1II/XhoI) Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L) Product is pBca9145-I716154 -------------------------------------------------------- kd007 Forward Bg1II for hemC ccaaaAGATCTatgataatgacggtaacaagc kd008 Reverse Xho1 for hemC gctagCTCGAGttaGGATCCtcatgccggggcgtctccgttatag kd009 Forward remove the EcoR1 site in hemC gccggttGAGTTCccgcaag kd010 Reverse remove the EcoR1 site in hemC cttgcggGAACTCaaccggc
