BerkiGEM2007-SamanthaConstructionFile11

From 2007.igem.org

(Difference between revisions)
 
Line 12: Line 12:
Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large) <br>
Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large) <br>
-
Digest pBca9145-I716209 (BglII/BglI, 1519+923bp, Large) <br>
+
Digest pBca9145-I716209 (BglII/BglI, 2898+923bp, Large) <br>
Product is pBca9145-I716217. <br>
Product is pBca9145-I716217. <br>

Latest revision as of 21:50, 12 June 2007

[rbs][ATG][lox][term][lox][ceaB]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site



Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large)
Digest pBca9145-I716209 (BglII/BglI, 2898+923bp, Large)
Product is pBca9145-I716216.


Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large)
Digest pBca9145-I716209 (BglII/BglI, 2898+923bp, Large)
Product is pBca9145-I716217.