BerkiGEM2007-VaibhaviConstructionFile2

From 2007.igem.org

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Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the SodC open reading frame from Escherichia coli K-12 MG1655
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Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the SodC open reading frame from Escherichia coli K-12 MG1655
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<pre>
<pre>
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PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI)
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PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI)
  Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L)
  Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L)
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  Product is pBca9145-VU1
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  Product is I716252
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VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc
VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc
VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc
VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc
</pre>
</pre>

Latest revision as of 20:35, 27 June 2007

Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the SodC open reading frame from Escherichia coli K-12 MG1655


PCR VU01F.sodC/VU02R.sodC on SodC		(553 bp, BglII/XhoI)
 Digest pBca9145-Bca1144 (Clone # 5)		(BglII/XhoI, 2063+910, L)
 Product is I716252
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VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc
VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc