Bologna University/Protocol validation measures
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Guido Costa (Talk  contribs) 
Guido Costa (Talk  contribs) 

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  +  Complete data set is in figure below.  
+  
+  [[Image:sito.jpg]]  
As we expected, the two mean values are close to each other, being the different spots from the same solution.  As we expected, the two mean values are close to each other, being the different spots from the same solution. 
Revision as of 16:17, 26 October 2007
We use bacteria with I763004 plasmid, since we know all of them beam fluorescence also when not inducted; we check that the OD value is 1.2. We then execute two different observations:
 ten different spots within the same slide (test A);
 five slides, and two spots each (10 total spots, test B).
For every spot, we take a picture and we elaborate it using the algorithm above, obtaining as output a normalized intensity value. Finally, for each of the two sets of values, we calculate mean and standard deviation; for the B test, we calculate both values either on the 10 intensities indistinctly (B1) and on the five slide means (B2). Here are the results:
Results  


Complete data set is in figure below.
As we expected, the two mean values are close to each other, being the different spots from the same solution.