Brown

From 2007.igem.org

(Difference between revisions)
Line 141: Line 141:
</p>
</p>
<!--In order to achieve this goal, we are constructing three constructs, each of which consists of a repressible, constitutively-on promoter attached to two repressors. Specifically, our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. Each of the three repressors are inactivated by one of three chemicals, the three inducer chemicals mentioned earlier. These three(arabinose, IPTG (Isopropyl β-D-1-thiogalactopyranoside) and Tetracycline, respectively), cause conformational changes in their respective repressor proteins which leads to gene expression. For example, in the presence of arabinose, AraC cannot repress pBAD so LacI and TetR are produced which in turn repress pTet and pLac.-->
<!--In order to achieve this goal, we are constructing three constructs, each of which consists of a repressible, constitutively-on promoter attached to two repressors. Specifically, our three constructs are pBAD->LacI->TetR, pLacI->AraC->TetR, and pTet->AraC->LacI, where AraC represses pBAD, LacI represses pLac and TetR represses pTet. Each of the three repressors are inactivated by one of three chemicals, the three inducer chemicals mentioned earlier. These three(arabinose, IPTG (Isopropyl β-D-1-thiogalactopyranoside) and Tetracycline, respectively), cause conformational changes in their respective repressor proteins which leads to gene expression. For example, in the presence of arabinose, AraC cannot repress pBAD so LacI and TetR are produced which in turn repress pTet and pLac.-->
 +
 +
http://brownigem.com/images/sponsors/pfizer_small.jpg
 +
http://brownigem.com/images/sponsors/labnet_small.jpg
 +
</td>
</td>
</tr>
</tr>

Revision as of 21:42, 24 October 2007

Brown University
Home Lead Sensor Tristable Switch Community Supplemental About us
Celluar Lead Sensor

Lead poisoning is a public health concern - there is lead in soil, paint, water, and dust. Lead Poisoning is often caused by ingesting contaminated drinking water, or soil. It can cause neurological and gastrointestinal disorders, especially among children.

The legal limit of lead in drinking water is 15 parts per billion.

Current ways of testing for lead either require expensive chemical lab analysis or involve inaccurate home kits.

A lead detector, based on E. Col, is cheap, sensitive, quick, and specific

Our system involves a Lead detecting promoter, an amplifier, and an output of GFP. We're also working on a system to remove false positives.

Tristable Switch

The Tri-Stable Toggle Switch represents a continuation on the theme of the Toggle Switch begun by Gardner, et al 2000. The switch produces three distinct and stable outputs in response to three distinct inputs. Our approach to designing the switch is based on quantitative measurements in a pioneering effort to establish a standardized means of designing and debugging biological systems.

http://brownigem.com/images/sponsors/pfizer_small.jpg http://brownigem.com/images/sponsors/labnet_small.jpg
  Links
Brown iGem on Openwetware
Brown iGem Official Website
Check out our Videos!
 News Updates
09.06.07
Brown University students love the Brown iGEM Booth at the Student Activities Fair!

09.04.07


Brown iGEM visits Pfizer HQ in Groton, Conneticut

08.28.07


Nanodrop donates ND-1000 Spectrophotometer and ND-3300 Fluorospectrophotometer to Brown iGEM

08.22.07


Brown iGEM Team presents its August Update to faculty and friends

08.20.07


Nanodrop it like it's hot! http://youtube.com/watch?v=pKT4Zrjeh6s

08.18.07


iGEM 2007 Meetup. Teams from MIT, BU, McGill, and Cold Spring Harbor presented their progress to each other and discussed the future of Synthetic Biology.

08.04.07


Brown iGEM presents summer research at the Brown University UTRA (Undergraduate Research) Symposium

07.30.07


Deepa and Tito gave a presentation to Brown Summer students and discussed the applications of synthetic biology

07.15.07


Brown iGEM presents June Update to faculty and friends of iGEM

07.03.07


The iGEM Team met up with Jody Hall's Biotechnology class on July 3rd. Afterwards we toured the lab and discussed the ethics of synthetic biology.

06.15.07


Brown iGEM presents June Update to Pfizer Team and Brown community

06.07.07


$25,000 grant from Pfizer for equipment purchases

06.01.07


Labnet sponsors Brown iGEM with vortex mixers, a microcentrifuge, pipettes, and autopipettes.