Chiba/Lab Notebook
From 2007.igem.org
(Difference between revisions)
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== FliC TEAM == | == FliC TEAM == | ||
- | + | *pUC19-FliC-Hisの作製 | |
- | + | **LINKER LIGATION | |
#pUC19-FliCをInverse PCR(五種類のF-Primer,R-Primer→15通りのPCR) | #pUC19-FliCをInverse PCR(五種類のF-Primer,R-Primer→15通りのPCR) | ||
##Check Size | ##Check Size |
Revision as of 05:25, 2 September 2007
Lux TEAM
FliC TEAM
- pUC19-FliC-Hisの作製
- LINKER LIGATION
- pUC19-FliCをInverse PCR(五種類のF-Primer,R-Primer→15通りのPCR)
- Check Size
- Digestion(EcoRI,NcoI)
- Zymo Clean(40μ溶出)→SAP(50μ系)→Zymo Clean(30μ溶出)
- Histag,Histag-antiをアニール
- Digestion(EcoRI,NcoI)
- Ligation
- Transformation
- miniprep
- Digestion test
- Sequence→FliCに6×His配列が挿入されていることを確認