ETHZ/Biology

From 2007.igem.org

(Difference between revisions)
(Fixed table, todo's, text.)
(System parts)
Line 145: Line 145:
| [http://partsregistry.org/Part:BBa_I739001 BBa_I739001]
| [http://partsregistry.org/Part:BBa_I739001 BBa_I739001]
|width="250"| constitutive subsystem
|width="250"| constitutive subsystem
-
| <partinfo>BBa_I739001 SpecifiedComponents</partinfo>
+
|width="157"| <partinfo>BBa_I739001 SpecifiedComponents</partinfo>
|-
|-
! 2
! 2
Line 212: Line 212:
|-
|-
|-
|-
-
! 1+2+3
+
!width="111"| 1+2+3
|width="285"| tetR + lacI + luxR production  
|width="285"| tetR + lacI + luxR production  
| [http://partsregistry.org/Part:BBa_I739013 BBa_I739013]
| [http://partsregistry.org/Part:BBa_I739013 BBa_I739013]
Line 261: Line 261:
|-
|-
!width="111"| 1'
!width="111"| 1'
-
| cI negative / tetR negative promoter
+
|width="285"| cI negative / tetR negative promoter
| [http://partsregistry.org/Part:BBa_I739102 BBa_I739102]
| [http://partsregistry.org/Part:BBa_I739102 BBa_I739102]
|width="250"| reporting subsystem
|width="250"| reporting subsystem
Line 304: Line 304:
|width="285"| PoC promoter
|width="285"| PoC promoter
| [http://partsregistry.org/Part:BBa_I739101 BBa_I739101]
| [http://partsregistry.org/Part:BBa_I739101 BBa_I739101]
-
| proof of concept, no part of the system  
+
|width="250"| proof of concept, no part of the system  
|width="157"| <partinfo>BBa_I739101 SpecifiedComponents</partinfo>
|width="157"| <partinfo>BBa_I739101 SpecifiedComponents</partinfo>
|-
|-

Revision as of 13:20, 20 October 2007

Eth zh logo 4.png
Main Page      Biology Pespective      Engineering Perspective      Meet the Team      Team Notes      Pictures!

Main Page      System Modeling      Simulations      System Implementation      Lab Notes      Meet the Team      Team Notes      Pictures!

.:: educatETH E.coli - Biology Perspective ::.

In this page, you can find an analysis of the function of our system and its relation to epigenetics, its biological design and a list of the parts that it consists of. Are you interested in constructing educatETH E.coli in your lab? Then under Lab Notes, you can find the ingredients and equipment we used, the electronic version of our lab notebook and a presentation of all the difficulties that we encountered. If you are also interested in how educatETH E.coli was simulated outside the lab, please visit the Engineering Perspective.


Contents

Introduction

educatETH E.coli is a system which can distinguish between aTc and IPTG based on a previous training phase conducted with the same chemicals and the help of AHL. It composes of three subsystems: the subsystem of constitutively produced proteins, the learning subsystem and the reporting subsystem. The constitutively produced proteins (lacI, TetR and LuxR) control the learning subsystem. At the core of the latter there exists a modified version of the toggle switch found in [1] with two operator sites, so that it only changes its state when both one of the two chemicals (aTc/IPTG) and AHL are present. As AHL is only present during the training phase, the toggle maintains its state during testing, and thus can “memorize”. In the reporting subsystem, four reporters allow supervision of both the chemical the system was trained with and of if the system recognizes the chemical it is being exposed to in the testing phase as one it has been trained with or not.


The complete system

The biological design of educatETH E.coli is presented on

Fig. 1: Gene interaction network of educatETH E.coli
. In the following, we will clarify the function of all depicted components. (Are you interested in how the complex system of Fig. 1 was modelled? Then visit the Engineering Perspective!)


Constitutive subsystem

The constitutively produced proteins of the system are LacI, TetR and LuxR. The LuxR part has a special function: when AHL is present, it forms a LuxR-AHL complex which acts on the learning subsystem (more on this later). At the moment, we will consider that AHL is absent and therefore LuxR cannot act on any subsystems. The TetR and lacI parts behave similarly: more specifically, the tetR protein in the absence of aTc inhibits the production of p22cII and LacI in the absence of IPTG inhibits the production of cI. When aTc is present, however, the p22cII production is no longer inhibited (and thus aTc is produced). Respectively, cI is produced when IPTG is present.

Learning subsystem

The learning subsystem is a toggle switch with two operator sites. The upper part of the toggle (cI production) has operator sites for the LuxR-AHL complex and p22cII (which has been in turn induced by aTc). The LuxR-AHL complex induces the cI production, whereas p22cII inhibits it. The lower part of the toggle (p22cII production) has operator sites for the LuxR-AHL comple and cI (which has been induced by IPTG). Similarly with the upper part, the LuxR-AHL complex induces the p22cII production and cI inhibits it. Therefore, the switch always requires the presence of the LuxR-AHL complex in order for it to operate. Its state depends on the presence of p22cII and cI into the system, which in curse was caused through the exposure of the system to aTc and IPTG.

Reporting subsystem

There are four reporters in the system. CFP and YFP are active during the training phase of the system and show which chemical the system is exposed to during training, whereas GFP and RFP are active during the testing phase and show if the system is exposed to the same chemical as in training or not. More specifically, the YFP protein production is regulated with help of two operator sites controlled by cI and aTc. cI inhibits YFP production and aTc induces it. Therefore, YFP is produced when the system is exposed to aTc. In a similar manner, the CFP production is produced when the system is exposed to IPTG. The GFP production is regulated with help of two operator sites controlled by lacI and .


System phases

The system operation is divided into two main phases: the training phase and the testing phase. The training phase itself is also subdivided into two phases: seeing and memorizing. During seeing, the system is first exposed to one of the two chemicals it is designed to recognize (aTc and IPTG). AHL is then added and the system’s internal toggle switch reaches a steady state. During memorizing, the chemical used during seeing is removed and only AHL is retained. This maintains the toggle switch to its acquired steady state, which is reported with YFP (if aTc was seen) or CFP (if IPTG was seen). During the testing phase, the system is exposed to any of the two chemicals (aTc or IPTG), with AHL present. By comparing its toggle switch state with the effect of the newly introduced chemical, the system shows a different response if it has previously been exposed to this chemical and reports with the same XFP as in the training phase (YFP for aTc, CFP for IPTG) or if it recognizes a different chemical and reports with a different XFP (GFP for trained with aTc and recognizing IPTG, RFP for trained with IPTG and recognizing aTc).The following table presents all possible paths that may be taken by the system during all phases of operation according to the external stimuli.

System phases
aTc IPTG AHL p22cII cI Reporting
Start
no input no no no no no no
Learning
Trained with aTc yes no no yes no YFP
Trained with IPTG no yes no no yes CFP
Memorizing
Trained with aTc yes no yes yes no YFP
Trained with IPTG no yes yes no yes CFP
Recognition
Trained with aTc
Tested with aTc
yes no yes yes no YFP
Trained with aTc
Tested with IPTG
no yes yes yes no GFP
Trained with IPTG
Tested with IPTG
no yes yes no yes CFP
Trained with IPTG
Tested with aTc
yes no yes no yes RFP

Further thoughts on the system phases

TODO: put Stefan's new text here. Text should be small.


System parts

educatETH E.coli consists of 11 parts that can be synthesized independently (want to know how this is done in the lab? Then visit our In the Lab page!) Please note that four of them (4,5 and 8,9) form together two functional system units. They have been separated to ensure comparable part lengths and thus enable easier introduction into plasmids.

Single System Parts
1 TetR production BBa_I739001 constitutive subsystem
2 LacI production BBa_I739002 constitutive subsystem
3 LuxR production BBa_I739003 constitutive subsystem
4 1st half of P22 cII / EYFP production BBa_I739004 reporting subsystem
5 2nd half of P22 cII / EYFP production BBa_I739005 reporting subsystem
6 cI production BBa_I739006 learning subsystem
7 P22 cII production BBa_I739007 learning subsystem
8 1st half of cI / ECFP production BBa_I739008 reporting subsystem
9 2nd half of cI / ECFP production BBa_I739009 reporting subsystem
10 RFP production BBa_I739010 reporting subsystem
11 GFP production BBa_I739011 reporting subsystem
Composite System Parts
1+2+3 tetR + lacI + luxR production BBa_I739013 constitutive subsystem
4+5 P22 cII + EYFP production BBa_I739015 reporting subsystem
8+9 cI + ECFP production BBa_I739016 reporting subsystem
(4+5)+(8+9) (P22 cII + EYFP) + (cI + ECFP) production BBa_I739017 reporting subsystem
6+7 cI + P22 cII production BBa_I739018 learning subsystem
10+11 RFP + GFP production BBa_I739019 reporting subsystem
(6+7)+(10+11) (cI + P22 cII) + (RFP + GFP) production BBa_I739020 learning/reporting subsystem


Double Promoters
1' cI negative / tetR negative promoter BBa_I739102 reporting subsystem
2' lacI negative / P22 cII negative promoter BBa_I739103 reporting subsystem
3' luxR/HSL positive / P22 cII negative promoter BBa_I739104 learning subsystem
4' luxR/HSL positive / cI negative promoter BBa_I739105 learning subsystem
5' tetR negative / P22 cII negative promoter BBa_I739106 reporting subsystem
6' cI negative / lacI negative promoter BBa_I739107 reporting subsystem
Proof of Concept
1" PoC promoter BBa_I739101 proof of concept, no part of the system
2" PoC intermediate BBa_I739014 proof of concept, no part of the system
3" PoC composite BBa_I739021 proof of concept, no part of the system


References

To Do

New

  • Update and correct parts in parts list. Write better in a table
  • Update and correct full system scheme
  • What is the proof of concept mentioned?
  • Try to improve table with system phases. It doesn't look so nice...
  • In the memorizing phase, is there color or not?
  • Check my terminology (operator sites etc)
  • Put Stefan's updated part on epigenetics
  • Fill in table completely, make it more reading-friendly