(Difference between revisions)
(Model Parameters)
(Model Parameters)
Line 150: Line 150:
| K<sub>cI</sub>
| K<sub>cI</sub>
* 8 [pM]
8 [pM]
* 50 [nM]
50 [nM]
| cI repressor dissociation constant
| cI repressor dissociation constant
* Ref. [12]
Ref. [12]
* starting with values of Ref. [6] and using Ref. [3]
starting with values of Ref. [6] and using Ref. [3]
| K<sub>p22cII</sub>
| K<sub>p22cII</sub>

Revision as of 12:34, 13 October 2007



Model Parameters

Parameter Value Description Comments Parameter Value Description Comments
c1max 0.01 [mM/h] max. transcription rate of constitutive promoter (per gene) promoter no. J23105; Estimate c2max 0.01 [mM/h] max. transcription rate of luxR-activated promoter (per gene) Estimate
lhi 25 high-copy plasmid number Estimate llo 5 low-copy plasmid number Estimate
a 1% basic production levels Estimate
Degradation constants
dlacI 2.31e-3 [per sec] degradation of lacI Ref. [10] dtetR 1e-5 [pro sec]/2.31e-3 [per sec] degradation of tetR

Ref. [9] Ref. [10]

dluxR 1e-3 - 1e-4 [per sec] degradation of luxR Ref: [6]
dcI 7e-4 [per sec] degradation of cI Ref. [7] dp22cII degradation of p22cII
dYFP 6.3e-3 [per min] degradation of YFP suppl. mat. to Ref. [8] corresponding to a half life of 110min dGFP 6.3e-3 [per min] degradation of GFP in analogy to YFP
dRFP 6.3e-3 [per min] degradation of RFP in analogy to YFP dCFP 6.3e-3 [per min] degradation of CFP in analogy to YFP
Dissociation constants
KlacI 0.1 - 1 [pM] lacI repressor dissociation constant Ref. [2] KIPTG 1.3 [µM] IPTG-lacI repressor dissociation constant Ref. [2]
KtetR 179 [pM] tetR repressor dissociation constant Ref. [1] KaTc 893 [pM] aTc-tetR repressor dissociation constant Ref. [1]
KluxR 55 - 520 [nM] luxR activator dissociation constant Ref: [6] KAHL 0.09 - 1 [µM] AHL-luxR activator dissociation constant Ref: [6]

8 [pM] 50 [nM]

cI repressor dissociation constant

Ref. [12] starting with values of Ref. [6] and using Ref. [3]

Kp22cII 0.577 [µM] p22cII repressor dissociation constant Ref. [11]. Note that they use a protein cII and we have p22cII. Does that match?
Hill cooperativity
nlacI 1 lacI repressor Hill cooperativity Ref. [5] nIPTG 2 IPTG-lacI repressor Hill cooperativity Ref. [5]
ntetR 3 tetR repressor Hill cooperativity Ref. [3] naTc 2 (1.5-2.5) aTc-tetR repressor Hill cooperativity Ref. [3]
nluxR 2 luxR activator Hill cooperativity Ref: [6] nAHL 1 AHL-luxR activator Hill cooperativity Ref. [3]
ncI 2 cI repressor Hill cooperativity Ref. [12] np22cII 4 p22cII repressor Hill cooperativity Ref. [11]. Note that they use a protein cII and we have p22cII. Does that match?


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