ETHZ/Simulation

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Contents

Protocol

Fig. 1: Flow diagram

Simulation of Test Cases

To test for all conditions we run timing simulations for all 4 different branches in the flow diagram of our protocol.

  • During the first 1000 minutes of the simulation we did not add any inducers to the system to check for the baseline production of proteins.
  • Between 1000 and 2000 minutes we added the inducer that should be learned and let the system reach steady state.
  • After the 2000<high>th</high> minute and 3000 minutes

Test 1

Fig 1: Test 1: learn and recognize IPTG

Test 2

Fig 2: Test 1: learn IPTG and get tested for aTc

Test 3

Fig 3: Test 1: learn and recognize aTc

Test 4

Fig 4: Test 1: learn aTc and get tested for IPTG

Sensitivity Analysis

For a system like our is is not enough to find a set of parameters for which it is working. To provide a stable performance also in the presence of noise and parameter missmatch we were interested in analysing the sensitivity of the system.

The results are plotted in Figure 5.

Fig. 5: Sensitivity analysis
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