ETHZ/labnotes

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=== Part/Plasmid Numberings ===
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The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.
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# [http://partsregistry.org/Part:BBa_B0034 B0034]
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# [http://partsregistry.org/Part:BBa_R0062 R0062]
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# [http://partsregistry.org/Part:BBa_R0053 R0053]
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# [http://partsregistry.org/Part:BBa_J23100 J23100]
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# [http://partsregistry.org/Part:BBa_J37033 J37033]
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# [http://partsregistry.org/Part:BBa_E0434 E0434]
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# [http://partsregistry.org/Part:BBa_B0015 B0015]
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# [http://partsregistry.org/Part:BBa_Q04400 Q04400]
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# [http://partsregistry.org/Part:BBa_R0010 R0010]
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# [http://partsregistry.org/Part:BBa_E0422 E0422]
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# [http://partsregistry.org/Part:BBa_R0040 R0040]
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# [http://partsregistry.org/Part:BBa_R0051 R0051]
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# [http://partsregistry.org/Part:BBa_Q04121 Q04121]
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# [http://partsregistry.org/Part:BBa_C0053 C0053]
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== Week 1 ==
== Week 1 ==

Revision as of 16:25, 10 August 2007

Part/Plasmid Numberings

The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.

  1. B0034
  2. R0062
  3. R0053
  4. J23100
  5. J37033
  6. E0434
  7. B0015
  8. Q04400
  9. R0010
  10. E0422
  11. R0040
  12. R0051
  13. Q04121
  14. C0053

Week 1

Date TODO's Completed People
Mon, 06. Aug. 2007
Tue, 07. Aug. 2007
Wed, 08. Aug. 2007
Thu, 09. Aug. 2007
Fri, 10. Aug. 2007
  • 7 working parts/plasmids (step after "DIGESTS"):
    (B0034, R0062, R0053, E0434, B0015, R0010, E0422)
  • 4 parts/plasmids minipreped:
    (R0040, R0051, Q04121, C0053)

Christos
Markus
Stefan

Sat, 11. Aug. 2007
Sun, 12. Aug. 2007
  • Prepare competent cells

Week 2

Date TODO's Completed People
Mon, 13. Aug. 2007
  • Transformations of J23100, J37033, Q04400, Q04510
  • Control Restrictions (step after "MINIPREP")
    R0040, R0051, Q04121, C0053
  • Ligation (step: "LINK ASSEMBLY"):
    R0053 + E0422
    R0010 + E0422
    R0010 + E0434
    S/P: R0053, R0010
    X/P: E0422, E0434
Tue, 14. Aug. 2007
Wed, 15. Aug. 2007
Thu, 16. Aug. 2007
Fri, 17. Aug. 2007
Sat, 18. Aug. 2007
Sun, 19. Aug. 2007
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