ETHZ/labnotes

From 2007.igem.org

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* Ligation (step: "LINK ASSEMBLY"): <br> R0053 + E0422 <br> R0010 + E0422 <br> R0010 + E0434 <br> S/P: R0053, R0010 <br> X/P: E0422, E0434                     
* Ligation (step: "LINK ASSEMBLY"): <br> R0053 + E0422 <br> R0010 + E0422 <br> R0010 + E0434 <br> S/P: R0053, R0010 <br> X/P: E0422, E0434                     
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Revision as of 08:31, 11 August 2007

Part/Plasmid Numberings

The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.

  1. B0034
  2. R0062
  3. R0053
  4. J23100
  5. J37033
  6. E0434
  7. B0015
  8. Q04400
  9. R0010
  10. E0422
  11. R0040
  12. R0051
  13. Q04121
  14. C0053

Week 1

Date TODO's Completed People
Mon, 06. Aug. 2007
Tue, 07. Aug. 2007
Wed, 08. Aug. 2007
Thu, 09. Aug. 2007
Fri, 10. Aug. 2007
  • 7 working parts/plasmids (step after "DIGESTS"):
    (B0034, R0062, R0053, E0434, B0015, R0010, E0422)
  • 4 parts/plasmids minipreped:
    (R0040, R0051, Q04121, C0053)

Christos
Markus
Stefan

Sat, 11. Aug. 2007
Sun, 12. Aug. 2007
  • Prepare competent cells

Christos
Joe
Martin

Week 2

Date TODO's Completed People
Mon, 13. Aug. 2007
  • Transformations of J23100, J37033, Q04400, Q04510
  • Control Restrictions (step after "MINIPREP")
    R0040, R0051, Q04121, C0053
  • Ligation (step: "LINK ASSEMBLY"):
    R0053 + E0422
    R0010 + E0422
    R0010 + E0434
    S/P: R0053, R0010
    X/P: E0422, E0434
* Tim (from 17:30 until it's done)
Martin
Tue, 14. Aug. 2007 * Tim (from 17:30 until it's done)
Martin
Wed, 15. Aug. 2007 * Tim (from 17:30 until it's done)
Martin
Thu, 16. Aug. 2007

Christos (I try to be the whole day around - will update that, after I take the day off)
Martin

Fri, 17. Aug. 2007 Martin
Sat, 18. Aug. 2007 Martin
Sun, 19. Aug. 2007 Martin