ETHZ/labnotes

From 2007.igem.org

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* Gel Electrophoresis of R0040, R0051, Q04121, C0053
* Gel Electrophoresis of R0040, R0051, Q04121, C0053
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|Morning Shift (start 9am): <br> Markus, Tim <br> <br> Evening Shift (start 5pm):<br> Martin, Christos
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|Morning Shift (9am-1pm?): <br> Markus, Tim <br> <br> Evening Shift (5pm-...):<br> Martin, Christos
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| Wed, 15. Aug. 2007           
| Wed, 15. Aug. 2007           

Revision as of 19:22, 13 August 2007

Part/Plasmid Numberings

The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.

  1. B0034
  2. R0062
  3. R0053
  4. J23100
  5. J37033
  6. E0434
  7. B0015
  8. Q04400
  9. R0010
  10. E0422
  11. R0040
  12. R0051
  13. Q04121
  14. C0053
  15. Q4510

Week 1

Date TODO's Completed People
Mon, 06. Aug. 2007
Tue, 07. Aug. 2007
Wed, 08. Aug. 2007
Thu, 09. Aug. 2007
Fri, 10. Aug. 2007
  • 7 working parts/plasmids (step after "DIGESTS"):
    (B0034, R0062, R0053, E0434, B0015, R0010, E0422)
  • 4 parts/plasmids minipreped:
    (R0040, R0051, Q04121, C0053)

Christos
Markus
Stefan

Sat, 11. Aug. 2007
Sun, 12. Aug. 2007
labwork cancelled

Christos
Joe
Martin

Week 2

Date TODO's Completed People
Mon, 13. Aug. 2007
start at 3 pm
  • Prepare competent cells
  • Transformations of J23100, J37033, Q04400, Q04510
  • Control Restrictions (step after "MINIPREP")
    R0040, R0051, Q04121, C0053
  • Overnight culture (Top 10 cells)
  • Control Restrictions (didn't work)
Martin
Markus
Christos
Tim
Tue, 14. Aug. 2007 Morning Shift:
  • Start Preparing competent cells for J23100, J37033, Q04400, Q04510
  • Control Restrictions (step after "MINIPREP")
    R0040, R0051, Q04121, C0053

Evening Shift:

  • Transformations of J23100, J37033, Q04400, Q04510
  • Gel Electrophoresis of R0040, R0051, Q04121, C0053
Morning Shift (9am-1pm?):
Markus, Tim

Evening Shift (5pm-...):
Martin, Christos
Wed, 15. Aug. 2007
  • Miniprep of competent cells (J23100, J37033, Q04400, Q04510)
  • Ligation (step: "LINK ASSEMBLY"):
    R0053 + E0422
    R0010 + E0422
    R0010 + E0434
    S/P: R0053, R0010
    X/P: E0422, E0434
* Tim (from 17:30 until it's done)
Martin
Christos (11:00-13:00, 18:00-00:00)
Thu, 16. Aug. 2007

Christos (11:00-13:00, 18:00-00:00)
Martin

Fri, 17. Aug. 2007 Martin
Nan (from 18:00 till 00:00)
Sat, 18. Aug. 2007 Martin
Nan
Sun, 19. Aug. 2007 Martin
Nan
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