The following are the numbering of the parts/plasmids that we use in the lab. Please use the same numberings if you label something when working in the lab. Extend the list for new concatenated parts.
Date
| TODO's
| Completed
| People
|
Mon, 06. Aug. 2007
|
|
| Sylke, Raphael, Stefan, Markus, Martin, Christos, Joe
|
Tue, 07. Aug. 2007
|
|
- Prepare competent cells for all parts
- Transformation of all the parts
| Sylke, Raphael, Stefan, Markus, Martin, Christos, Joe
|
Wed, 08. Aug. 2007
|
|
- Preparing the grown cultures (12) for the MINIPREP
(o/n cultures)
| Raphael, Stefan
|
Thu, 09. Aug. 2007
|
|
- MINIPREP of the grown (10) o/n cultures
- Gelelectrophoresis of the grown cultures (step: 0.8% Agarose)
| Raphael, Stefan, Martin, Christos, Joe
|
Fri, 10. Aug. 2007
|
|
- 7 working parts/plasmids (step after "DIGESTS"):
(B0034, R0062, R0053, E0434, B0015, R0010, E0422)
- 4 parts/plasmids minipreped:
(R0040, R0051, Q04121, C0053)
|
Christos Markus Stefan
|
Sat, 11. Aug. 2007
|
| no labwork
|
|
Sun, 12. Aug. 2007
|
| labwork cancelled
|
|
Date
| TODO's
| Completed
| People
|
Mon, 13. Aug. 2007 start at 3 pm
|
- Prepare competent cells
- Transformations of J23100, J37033, Q04400, Q04510
- Control Restrictions (step after "MINIPREP")
R0040, R0051, Q04121, C0053
|
- o/n culture (E.Coli Top10)
- Control Restrictions (didn't work)
| Martin Markus Christos Tim
|
Tue, 14. Aug. 2007
| Morning Shift:
- Start Preparing competent cells (for J23100, J37033, Q04400, Q04510)
Evening Shift:
- Transformations of J23100, J37033, Q04400, Q04510
| Morning Shift:
- Prepared competent cells (stored in the -80°C freezer in the basement)
Evening Shift:
- Transformation of J23100, J37033, Q04400, Q04510 and R0040, R0051, Q04121, C0053 (in the 37°C incubator until Wednesday)
- Prepared new Liquid LB, LB Agar (both in the autoclave), Agarose Gel with concentrations of 0.8% and 2.4%
| Morning Shift (9am-1pm?): Markus, Tim Evening Shift (5pm-...): Martin, Christos
|
Wed, 15. Aug. 2007
|
- Ligation (step: "LINK ASSEMBLY"):
R0053 + E0422 R0010 + E0422 R0010 + E0434 S/P: R0053, R0010 X/P: E0422, E0434
|
- Ligation didn't work due to bad quality of enzymes (probably)
| From 12: Martin, Markus
From 5pm: Christos , Tim (?)
|
Thu, 16. Aug. 2007
|
- Miniprep (J23100, J37033, Q04400, Q04510, R0040, R0051, Q04121, C0053)
- Transformation of #13 and #14
|
|
Martin
|
Fri, 17. Aug. 2007
|
|
| Martin Nan (from 18:00 till 00:00)
|
Sat, 18. Aug. 2007
|
|
| Martin Nan
|
Sun, 19. Aug. 2007
|
|
| Martin Nan
|