http://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&feed=atom&action=historyFreiburg07/labnotes - Revision history2024-03-28T19:05:53ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44358&oldid=prevPhilipp: /* Do, 04th Okt. 2007 */2007-10-26T20:05:32Z<p><span class="autocomment">Do, 04th Okt. 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Sequences for the synthesis prepared<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Sequences for the synthesis prepared<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>...At that point of time we had to <del class="diffchange diffchange-inline">stop </del>our lab-work and focus on the theoretical planing and uoploading of our parts and their synthesis by GeneArt...</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''''</ins>...At that point of time we had to <ins class="diffchange diffchange-inline">leave </ins>our <ins class="diffchange diffchange-inline">-not really finished- </ins>lab-work and focus on the theoretical planing and uoploading of our parts and their synthesis by GeneArt...<ins class="diffchange diffchange-inline">'''''<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[https://2007.igem.org/Freiburg BACK]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[https://2007.igem.org/Freiburg BACK]</div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44293&oldid=prevPhilipp: /* Do, 04th Okt. 2007 */2007-10-26T19:59:30Z<p><span class="autocomment">Do, 04th Okt. 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Sequences for the synthesis prepared<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Sequences for the synthesis prepared<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">...At that point of time we had to stop our lab-work and focus on the theoretical planing and uoploading of our parts and their synthesis by GeneArt...</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[https://2007.igem.org/Freiburg BACK]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[https://2007.igem.org/Freiburg BACK]</div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44249&oldid=prevPhilipp: /* So, 23th Sept. 2007 */2007-10-26T19:54:53Z<p><span class="autocomment">So, 23th Sept. 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-digestion(EcoRI,SpeI) of the CFP vectors repeated, gel extraktion (only 5 bands instead of 2!?)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-digestion(EcoRI,SpeI) of the CFP vectors repeated, gel extraktion (only 5 bands instead of 2!?)<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-approach of an new over night culture for this plasmid (glycerol stock from Urs)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-approach of an new over night culture for this plasmid (glycerol stock from Urs)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-approach of another growth test (cross-check with the dhfr-winzip construct) in M9 for dhfr1-4calmo4-dhfr2 construct<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-approach of another growth test (cross-check with the dhfr-winzip construct <ins class="diffchange diffchange-inline">for "background"-measurement</ins>) in M9 for dhfr1-4calmo4-dhfr2 construct<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Mo, 24th Sept. 2007 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Mo, 24th Sept. 2007 ==</div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44236&oldid=prevPhilipp: /* So, 23th Sept. 2007 */2007-10-26T19:54:12Z<p><span class="autocomment">So, 23th Sept. 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-digestion(EcoRI,SpeI) of the CFP vectors repeated, gel extraktion (only 5 bands instead of 2!?)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-digestion(EcoRI,SpeI) of the CFP vectors repeated, gel extraktion (only 5 bands instead of 2!?)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-approach of an new over night culture for <del class="diffchange diffchange-inline">tis </del>plasmid (glycerol stock from Urs)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-approach of an new over night culture for <ins class="diffchange diffchange-inline">this </ins>plasmid (glycerol stock from Urs)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-approach of another growth test (<del class="diffchange diffchange-inline">crosscheck </del>with the dhfr-winzip construct) in M9 for dhfr1-4calmo4-dhfr2 construct<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-approach of another growth test (<ins class="diffchange diffchange-inline">cross-check </ins>with the dhfr-winzip construct) in M9 for dhfr1-4calmo4-dhfr2 construct<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Mo, 24th Sept. 2007 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Mo, 24th Sept. 2007 ==</div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44206&oldid=prevPhilipp: /* Fr, 24th August 2007 */2007-10-26T19:52:09Z<p><span class="autocomment">Fr, 24th August 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Dario (12-16 o´clock):<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Dario (12-16 o´clock):<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Counting of colonies on plates of bl_calmo2,4,6_b2 with different concentrations of Ca2+,<br> Amp und IPTG.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Counting of colonies on plates of bl_calmo2,4,6_b2 with different concentrations of Ca2+,<br> Amp und IPTG.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- PheBo <del class="diffchange diffchange-inline">loding </del>buffer for the protein purification of b1_calmo4_b2 in XL-1 was changed.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- PheBo <ins class="diffchange diffchange-inline">loading </ins>buffer for the protein purification of b1_calmo4_b2 in XL-1 was changed.<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Natalia (9.00-11.00 o´clock)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Natalia (9.00-11.00 o´clock)<br></div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44198&oldid=prevPhilipp: /* Thu, 23th August 2007 */2007-10-26T19:51:37Z<p><span class="autocomment">Thu, 23th August 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b>4. PCR</b><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b>4. PCR</b><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - <del class="diffchange diffchange-inline">With </del>Fhy1, Phy A and Fhy <del class="diffchange diffchange-inline">is everything working </del>(Digestions</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - <ins class="diffchange diffchange-inline"> everything is working out with </ins>Fhy1, Phy A and Fhy <ins class="diffchange diffchange-inline"> </ins>(Digestions</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>and PCR)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>and PCR)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Now <del class="diffchange diffchange-inline">must </del>Fhy1 with YFP/YFP with FHY/ PHy A with CFP merged<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Now <ins class="diffchange diffchange-inline"> </ins>Fhy1 with YFP/YFP with FHY/ PHy A with CFP <ins class="diffchange diffchange-inline">must be </ins>merged<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Next experiments. FRET ( Activity test)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Next experiments. FRET ( Activity test)<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Purification of the expression culture of b1_calmo4,4_b2 clone 1 in XL-1 still</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Purification of the expression culture of b1_calmo4,4_b2 clone 1 in XL-1 still</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>in process.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>in process.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- <del class="diffchange diffchange-inline">Assinting </del>Natalia with the Calmo-constructs with different concentration</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- <ins class="diffchange diffchange-inline">Assisting </ins>Natalia with the Calmo-constructs with different concentration</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>of&nbsp; Ca2+, IPTG und Amp</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>of&nbsp; Ca2+, IPTG und Amp</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44173&oldid=prevPhilipp: /* Thu, 23th August 2007 */2007-10-26T19:49:07Z<p><span class="autocomment">Thu, 23th August 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Transformation in BL21 with pREP4<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Transformation in BL21 with pREP4<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><b>2. <del class="diffchange diffchange-inline">Bei </del>DHFR1_wz_DHFR2</b><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><b>2. <ins class="diffchange diffchange-inline">With </ins>DHFR1_wz_DHFR2</b><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Sequencing: Exchanges in a few bases, but not in&nbsp; DHFR.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&nbsp;&nbsp;&nbsp; - Sequencing: Exchanges in a few bases, but not in&nbsp; DHFR.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Possible mistake: Plasmid is good, the problem is the plasmid map<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Possible mistake: Plasmid is good, the problem is the plasmid map<br></div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44141&oldid=prevPhilipp: /* Mo, 01st Okt. 2007 */2007-10-26T19:46:31Z<p><span class="autocomment">Mo, 01st Okt. 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Digestion with KpnI/SpeI, gel running, gel extraktion, determination of the concentration from Calmodulin (Insert) and blac1-winzip-blac2(Vektor)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Digestion with KpnI/SpeI, gel running, gel extraktion, determination of the concentration from Calmodulin (Insert) and blac1-winzip-blac2(Vektor)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-new approach <del class="diffchange diffchange-inline">dof </del>the M9-Ca2+ dilution series<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-new approach <ins class="diffchange diffchange-inline">of </ins>the M9-Ca2+ dilution series<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Di, 02nd Okt. 2007 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Di, 02nd Okt. 2007 ==</div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44137&oldid=prevPhilipp: /* Mi, 26th Sept. 2007 */2007-10-26T19:46:09Z<p><span class="autocomment">Mi, 26th Sept. 2007</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-spin prep of the dhfr-4calmo over night culture<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-spin prep of the dhfr-4calmo over night culture<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-SDS gel evaluated and scaned<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-SDS gel evaluated and scaned<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">peptidinformations </del>corrected...<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-corrected <ins class="diffchange diffchange-inline">peptide-informations</ins>...<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Do, 27th Sept. 2007 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Do, 27th Sept. 2007 ==</div></td></tr>
</table>Philipphttp://2007.igem.org/wiki/index.php?title=Freiburg07/labnotes&diff=44129&oldid=prevPhilipp: /* So, 23th Sept. 2007 */2007-10-26T19:45:08Z<p><span class="autocomment">So, 23th Sept. 2007</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:45, 26 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-digestion(EcoRI,SpeI) of the CFP vectors repeated, gel extraktion (only 5 bands instead of 2!?)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-digestion(EcoRI,SpeI) of the CFP vectors repeated, gel extraktion (only 5 bands instead of 2!?)<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-approach of an new over night culture for tis plasmid (glycerol stock from Urs)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-approach of an new over night culture for tis plasmid (glycerol stock from Urs)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-approach of another growth test (<del class="diffchange diffchange-inline">bzw. der </del>crosscheck with the dhfr-winzip construct) in M9 for dhfr1-4calmo4-dhfr2 construct<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-approach of another growth test (crosscheck with the dhfr-winzip construct) in M9 for dhfr1-4calmo4-dhfr2 construct<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Mo, 24th Sept. 2007 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Mo, 24th Sept. 2007 ==</div></td></tr>
</table>Philipp