Freiburg07/report light sensor

From 2007.igem.org

(Difference between revisions)
Line 14: Line 14:
{|  
{|  
|-  
|-  
-
| class="taxo-image" | [[Image:inaktivtest.jpg|thumb|398px|'''Picture:''' DHFR-Calmodulin-Construct, inactive state; no calcine bound]]
+
| class="taxo-image" | [[Image:PhyA_Fhy1_cycle.png|thumb|398px| PhyA - Fhy1 Interaction and light cycle]]
|}</div>
|}</div>

Revision as of 13:46, 23 October 2007

Contents

Towards optical signal processing in E. coli

iGEM team Freiburg

Introduction

Phytochrome A (PhyA) is a member of the phytochrome family of red/far-red photo-
receptors. These photoreceptors are involved in a wide range of developmental
responses in plants. Fhy1 is a protein which binds PhyA under light conditions,
enable us to use this protein-protein interaction. In plants Fhy1 is responsable
for nuclear accumulation of PhyA in case of light exposure.


PhyA - Fhy1 Interaction and light cycle

Materials and Methods

Cloning of constructs:

PhyA Insert was gained by PCR from plasmid D153ah-phyA using 5´ (5´-TATCGACGAA TTCGCGGCCG CTTCTAGAAA GAGGAGAAAT
TAACTATGTC AGGCTCTAGG CCGAC-3´) and 3´ (5´- AACGATCACT AGTTCCGCCG TGATGGTGAT GGTGGCCTCC GTTATCGAGT TCCACCTCCT TA-3´) primers.
Fhy1 Insert1 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCGATTACA TCTATGGGAC TCAGA-3´) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.
Fhy1 Insert2 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCAGTAGTA ATGCTGCTAA GTTTGT-3`) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.

Results

Discussion

References

BACK