Freiburg07/report light sensor

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<h2>Introduction</h2>
<h2>Introduction</h2>
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Phytochrome A (PhyA) is a member of the phytochrome family of red/far-red photo-<br>
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Phytochrome A (PhyA) is a member of the phytochrome family of red/far-red photoreceptors. These photoreceptors are involved in a wide range of developmental responses in plants. Fhy1 is a protein which binds PhyA  under light conditions, enable us to use this protein-protein interaction. In plants Fhy1 is responsable for nuclear accumulation of PhyA in case of light exposure.
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receptors. These photoreceptors are involved in a wide range of developmental<br>
+
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responses in plants. Fhy1 is a protein which binds PhyA  under light conditions,<br>
+
-
enable us to use this protein-protein interaction. In plants Fhy1 is responsable<br>
+
-
for nuclear accumulation of PhyA in case of light exposure.
+
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Revision as of 13:52, 23 October 2007

Contents

Towards optical signal processing in E. coli

iGEM team Freiburg

Introduction

Phytochrome A (PhyA) is a member of the phytochrome family of red/far-red photoreceptors. These photoreceptors are involved in a wide range of developmental responses in plants. Fhy1 is a protein which binds PhyA under light conditions, enable us to use this protein-protein interaction. In plants Fhy1 is responsable for nuclear accumulation of PhyA in case of light exposure.

PhyA - Fhy1 Interaction and light cycle

Materials and Methods

Cloning of constructs:

PhyA Insert was gained by PCR from plasmid D153ah-phyA using 5´ (5´-TATCGACGAA TTCGCGGCCG CTTCTAGAAA GAGGAGAAAT
TAACTATGTC AGGCTCTAGG CCGAC-3´) and 3´ (5´- AACGATCACT AGTTCCGCCG TGATGGTGAT GGTGGCCTCC GTTATCGAGT TCCACCTCCT TA-3´) primers.
Fhy1 Insert1 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCGATTACA TCTATGGGAC TCAGA-3´) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.
Fhy1 Insert2 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCAGTAGTA ATGCTGCTAA GTTTGT-3`) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.

Results

Discussion

References

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