Imperial/Cell-Free/Contribution

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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Whatis" title=""><span>What is Cell-Free?</span></a></li>
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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Comparison" title=""><span>Advantages of CFS</span></a></li>
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    <li><a class="current" href="https://2007.igem.org/Imperial/Cell-Free/Contribution" title=""><span>Our Contributions</span></a></li>
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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Characterisation" title=""><span>Characterisation</span></a></li>
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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Applications" title=""><span>CFS Applications</span></a></li>
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== Cell-Free: Our Contribution ==
== Cell-Free: Our Contribution ==

Revision as of 19:53, 26 October 2007



Cell-Free: Our Contribution

In vitro Expression

  • Attempted to make S30 E. coli cell extract and feeding solution
  • Successfully used commercial S30 E. coli cell extract and feeding solution from Promega for expression of GFP
  • Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
  • Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution


Vesicles Formation

  • Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
  • Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
  • Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
  • Attempting to find suitable pore proteins to prolong vesicle and expression lifespan


Documentation on Registry of Parts

Documented our findings with the following Registry Additions:

Cell-Free Systems



<< Cell-Free vs. Cell | Our Contribution | Characterisation >>