http://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&feed=atom&action=historyImperial/Cell by Date/Conclusion - Revision history2024-03-29T13:18:24ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=47263&oldid=prevMaira at 02:36, 27 October 20072007-10-27T02:36:39Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:36, 27 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:IC07navmenu}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:IC07navmenu}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br clear="all"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /></div></td></tr>
</table>Mairahttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=47248&oldid=prevJec105: /* Cell by Date: Conclusion */2007-10-27T02:35:08Z<p><span class="autocomment">Cell by Date: Conclusion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:35, 27 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 38:</td>
<td colspan="2" class="diff-lineno">Line 38:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|'''<font color=red><del class="diffchange diffchange-inline">Future work</del>- DSred Express can be substituted for GFPmut3b</font>''' </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|'''<font color=red><ins class="diffchange diffchange-inline">Not Determined</ins>- DSred Express can be substituted for GFPmut3b</font>''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td></tr>
</table>Jec105http://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=46619&oldid=prevAlexander.wong at 01:23, 27 October 20072007-10-27T01:23:53Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:23, 27 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 58:</td>
<td colspan="2" class="diff-lineno">Line 58:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Cell By Date is a great example of how synthetic biology can be integrated into our daily lives. When fully developed it can mimic the function of inducstrial level devices in the food industry at a fraction of the cost. Being a reporter of a break in the cold chain, CBD, can inform the end consumer whether the product he is buying has been exposed to abnormal storage conditions.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">This </del>is <del class="diffchange diffchange-inline">best explained with an example</del>. <del class="diffchange diffchange-inline">Imagine your local supermarket expecting a delivery of frozen lamb steaks. The delivery van arrives, </del>the <del class="diffchange diffchange-inline">goods are unloaded and placed on the shelves awaiting for their first victim </del>to <del class="diffchange diffchange-inline">arrive. Victim because what </del>the <del class="diffchange diffchange-inline">supermarket manager and </del>the <del class="diffchange diffchange-inline">end consumer are not aware </del>of <del class="diffchange diffchange-inline">is that during their transport </del>the <del class="diffchange diffchange-inline">van fridge broke down and thus </del>the <del class="diffchange diffchange-inline">steaks were defrosted</del>. <del class="diffchange diffchange-inline">The vab driver however fixed </del>the <del class="diffchange diffchange-inline">fault</del>, <del class="diffchange diffchange-inline">refroze </del>the <del class="diffchange diffchange-inline">meat </del>and <del class="diffchange diffchange-inline">told nothing upon his arrival to </del>the <del class="diffchange diffchange-inline">supermarket. The whole incident will be kept under cover until the first cases of food poisoning arise from the comprimised lamb </del>meat.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Cell by Date </ins>is <ins class="diffchange diffchange-inline">exemplary in how Synthetic Biology can be integrated into our everyday lives</ins>. <ins class="diffchange diffchange-inline">If </ins>the <ins class="diffchange diffchange-inline">system was properly coupled </ins>to the <ins class="diffchange diffchange-inline">spoilage of meat, it could mimic </ins>the <ins class="diffchange diffchange-inline">function </ins>of <ins class="diffchange diffchange-inline">industrial level devices in </ins>the <ins class="diffchange diffchange-inline">meat industry, all at a fraction of </ins>the <ins class="diffchange diffchange-inline">cost</ins>. <ins class="diffchange diffchange-inline">By constantly monitoring for a break in </ins>the <ins class="diffchange diffchange-inline">cold chain</ins>, <ins class="diffchange diffchange-inline">CBD will inform consumers of any mishandling of </ins>the <ins class="diffchange diffchange-inline">product, </ins>and <ins class="diffchange diffchange-inline">whether or not </ins>the meat <ins class="diffchange diffchange-inline">has been exposed to inappropriate storage/transport conditions</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">This is were </del>CBD <del class="diffchange diffchange-inline">comes in handy</del>. <del class="diffchange diffchange-inline">By embedding a sticker of </del>CBD <del class="diffchange diffchange-inline">on packaged goods, the consumer can check at any time whether the product quality </del>has been <del class="diffchange diffchange-inline">comprimised by exposure to ubnormally high temperatures. Just a glance at the sticker and its bright fluorescent colour will inform the buyer of a potential risk. That is if the quality control expert of the supermarket does not spot it first</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br clear="all"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:IC2007 </ins>CBD <ins class="diffchange diffchange-inline">packaging</ins>.<ins class="diffchange diffchange-inline">jpg|thumb|left|430px|'''</ins>CBD <ins class="diffchange diffchange-inline">deactivated''' - Cold chain </ins>has <ins class="diffchange diffchange-inline">not </ins>been <ins class="diffchange diffchange-inline">disrupted]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:IC2007 CBD packaging2</ins>.<ins class="diffchange diffchange-inline">jpg|thumb|right|430px|'''CBD activated''' (Red Glow) - Cold chain has been disrupted]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Even though not fully characterised</del>, <del class="diffchange diffchange-inline">promising progress has been made </del>and <del class="diffchange diffchange-inline">shows that even without tweaking </del>the <del class="diffchange diffchange-inline">initial design</del>, Cell by Date <del class="diffchange diffchange-inline">has a great potential just waiting to </del>be <del class="diffchange diffchange-inline">exploited</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On a bigger note, Cell by Date could be extended beyond the meat industry unto cold chain management iteself, targetting, in particular, pharmaceuticals. This is evident in the global supply of vaccines, especially to those in distant clinics in hot climates which are served by poorly developed transport networks. Under these circumstances, a break in the cold chain could severely compromise the efficacy of the vaccines</ins>, and <ins class="diffchange diffchange-inline">disrupt </ins>the <ins class="diffchange diffchange-inline">administering of a vaccination campaign that could bring more harm than good. As many vaccines are particularly temperature-sensitive</ins>, Cell by Date <ins class="diffchange diffchange-inline">may in fact </ins>be <ins class="diffchange diffchange-inline">more appropriately suited to such products that require a more sensitiive monitoring device like CBD</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>= Future Work =</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In conclusion, even though not fully characterised, promising progress has been made, in which our system shows that even without tweaking the initial design, Cell by Date has a great potential that is just waiting to be exploited.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">=</ins>= Future Work <ins class="diffchange diffchange-inline">=</ins>=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Extending the life of the reaction===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Extending the life of the reaction===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A major <del class="diffchange diffchange-inline">constrain </del>of CBD is its short lifetime. The cell-free extract <del class="diffchange diffchange-inline">used </del>currently can only support it for a maximum of a few days or for the production of 12mg of protein whichever comes first. A good <del class="diffchange diffchange-inline">amount of research can go into developing </del>cell <del class="diffchange diffchange-inline">extract </del>that <del class="diffchange diffchange-inline">can be stored at various temperatures for months and yet have a good performance and produce a fai amount </del>of <del class="diffchange diffchange-inline">protein. The more fluorescent protein produced </del>the <del class="diffchange diffchange-inline">better </del>the <del class="diffchange diffchange-inline">visible signal CBD will provide</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A major <ins class="diffchange diffchange-inline">constraint </ins>of CBD is its short lifetime. The cell-free extract <ins class="diffchange diffchange-inline">that is </ins>currently <ins class="diffchange diffchange-inline">used </ins>can only support it for a maximum of a few days<ins class="diffchange diffchange-inline">, </ins>or for the production of 12mg of protein whichever comes first. A good <ins class="diffchange diffchange-inline">approach to further this project would thus be to develop and optimise the </ins>cell <ins class="diffchange diffchange-inline">extracts such </ins>that <ins class="diffchange diffchange-inline">is is capable </ins>of <ins class="diffchange diffchange-inline">expression over </ins>the <ins class="diffchange diffchange-inline">lifespan of </ins>the <ins class="diffchange diffchange-inline">product that it is monitoring</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Packaging===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Packaging===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Being cell-free, <del class="diffchange diffchange-inline">Cell by Date, makes </del>it easier <del class="diffchange diffchange-inline">to </del>be embedded on food packaging<del class="diffchange diffchange-inline">. This </del>calls for an efficient packaging method that is transparent (for the person to be able to see the fluorescent protein) but also well isolated from the actual food source (to avoid contamination). <del class="diffchange diffchange-inline">There are also 2 </del>important factors <del class="diffchange diffchange-inline">that </del>need to be taken into consideration. The first is the fact that the cell extract requires oxygen for the <del class="diffchange diffchange-inline">reaction to occur</del>. <del class="diffchange diffchange-inline">What </del>is <del class="diffchange diffchange-inline">not known is how much is needed. This raises </del>the <del class="diffchange diffchange-inline">issue whether sealing CBD in </del>a <del class="diffchange diffchange-inline">package with </del>limited oxygen <del class="diffchange diffchange-inline">will prevent the reaction from occuring</del>. The other factor is evaporation. <del class="diffchange diffchange-inline">The smaller the sample tested</del>, <del class="diffchange diffchange-inline">the more the evaporation there was </del>and the <del class="diffchange diffchange-inline">quicker </del>the reaction mixture <del class="diffchange diffchange-inline">vanished</del>. The packaging must therefore sustain the volume of the reaction for the lifespan of the <del class="diffchange diffchange-inline">food </del>product.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Packaging is also another aspect of Cell by Date that can be further developed. </ins>Being cell-free, it <ins class="diffchange diffchange-inline">is generally </ins>easier <ins class="diffchange diffchange-inline">for CBD </ins>be embedded on food packaging<ins class="diffchange diffchange-inline">, which </ins>calls for an efficient packaging method that is transparent (for the person to be able to see the fluorescent protein)<ins class="diffchange diffchange-inline">, </ins>but also well isolated from the actual food source (to avoid contamination). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><br clear="all"></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[Image:IC2007 CBD packaging.jpg|thumb|left|430px|'''CBD deactivated''' - Cold chain has not been disrupted]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Two </ins>important factors <ins class="diffchange diffchange-inline">however </ins>need to be taken into consideration. The first is the fact that the cell extract requires oxygen for the <ins class="diffchange diffchange-inline">maturation of GFP</ins>. <ins class="diffchange diffchange-inline">It </ins>is <ins class="diffchange diffchange-inline">indetermined of </ins>the <ins class="diffchange diffchange-inline">amount of oxygen required at present, and more tests would need to be performed to understand the conditions of </ins>a limited oxygen <ins class="diffchange diffchange-inline">environment</ins>. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[Image:IC2007 CBD packaging2.jpg|thumb|right|430px|'''CBD activated''' (Red Glow) - Cold chain has been disrupted]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The other factor is evaporation. <ins class="diffchange diffchange-inline">This is a huge factor</ins>, and <ins class="diffchange diffchange-inline">posed a lot of problems for us over </ins>the <ins class="diffchange diffchange-inline">course of experimentation as it affected the performance of </ins>the reaction mixture. The packaging must therefore <ins class="diffchange diffchange-inline">be able to </ins>sustain the volume of the reaction <ins class="diffchange diffchange-inline">mixture </ins>for the lifespan of the product <ins class="diffchange diffchange-inline">as to ensure its efficacy as a temperature-time integrator</ins>.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:IC2007 CBD packaging3.jpg|thumb|left|430px|'''CBD deactivated''' - Cold chain has not been disrupted]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:IC2007 CBD packaging3.jpg|thumb|left|430px|'''CBD deactivated''' - Cold chain has not been disrupted]]</div></td></tr>
</table>Alexander.wonghttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=45844&oldid=prevDirkvs: /* Cell by Date: Conclusion */2007-10-26T23:15:54Z<p><span class="autocomment">Cell by Date: Conclusion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:15, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 42:</td>
<td colspan="2" class="diff-lineno">Line 42:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||'''<font color=red><del class="diffchange diffchange-inline">No activation </del>energy of system was calculated to be 1.5kJ/mol</font>''' </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||'''<font color=red><ins class="diffchange diffchange-inline">Activation </ins>energy of system was calculated to be 1.5kJ/mol</font>''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Health Regulations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Health Regulations</div></td></tr>
</table>Dirkvshttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=45135&oldid=prevAnthony L: /* Cell by Date: Conclusion */2007-10-26T21:44:17Z<p><span class="autocomment">Cell by Date: Conclusion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:44, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 57:</td>
<td colspan="2" class="diff-lineno">Line 57:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Conclusion still to do</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cell By Date is a great example of how synthetic biology can be integrated into our daily lives. When fully developed it can mimic the function of inducstrial level devices in the food industry at a fraction of the cost. Being a reporter of a break in the cold chain, CBD, can inform the end consumer whether the product he is buying has been exposed to abnormal storage conditions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cell By Date is a great example of how synthetic biology can be integrated into our daily lives. When fully developed it can mimic the function of inducstrial level devices in the food industry at a fraction of the cost. Being a reporter of a break in the cold chain, CBD, can inform the end consumer whether the product he is buying has been exposed to abnormal storage conditions.</div></td></tr>
</table>Anthony Lhttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=44658&oldid=prevPeixuan at 20:41, 26 October 20072007-10-26T20:41:28Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:41, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 29:</td>
<td colspan="2" class="diff-lineno">Line 29:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Inputs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Inputs</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Isothermal conditions between 0 & 40 C</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Isothermal conditions between 0 & 40 C</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline"><center> </del>'''<font color=green>4&deg;C shows negligible activity while 37&deg;C shows highest fluorescence <del class="diffchange diffchange-inline"></center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">|</ins>'''<font color=green>4&deg;C shows negligible activity while 37&deg;C shows highest fluorescence </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Dynamic conditions eg. steps & ramps</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Dynamic conditions eg. steps & ramps</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<del class="diffchange diffchange-inline"><center></del>'''<font color=green>The system responded to stepping of temperature up and down</font>''' <del class="diffchange diffchange-inline"></center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||'''<font color=green>The system responded to stepping of temperature up and down</font>''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline"><center></del>'''<font color=red>Future work- DSred Express can be substituted for GFPmut3b</font>''' <del class="diffchange diffchange-inline"></center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|'''<font color=red>Future work- DSred Express can be substituted for GFPmut3b</font>''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<del class="diffchange diffchange-inline"><center></del>'''<font color=red>No activation energy of system was calculated to be 1.5kJ/mol</font>''' <del class="diffchange diffchange-inline"></center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||'''<font color=red>No activation energy of system was calculated to be 1.5kJ/mol</font>''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Health Regulations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Health Regulations</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 50:</td>
<td colspan="2" class="diff-lineno">Line 50:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Response Time</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Response Time</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have a response time under 1 hour</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have a response time under 1 hour</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<del class="diffchange diffchange-inline"><center></del>'''<font color=green>Yes system increases significantly after 1 hour</font>''' <del class="diffchange diffchange-inline"></center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||'''<font color=green>Yes system increases significantly after 1 hour</font>''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Lifespan</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Lifespan</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must have a shelf life of 7 days</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must have a shelf life of 7 days</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<del class="diffchange diffchange-inline"><center></del>'''<font color=red>Not Determined- Packaging method that prevents evaporation</font>''' <del class="diffchange diffchange-inline"></center></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||'''<font color=red>Not Determined- Packaging method that prevents evaporation</font>''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td></tr>
</table>Peixuanhttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=44648&oldid=prevPeixuan: /* Cell by Date: Conclusion */2007-10-26T20:40:01Z<p><span class="autocomment">Cell by Date: Conclusion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:40, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 29:</td>
<td colspan="2" class="diff-lineno">Line 29:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Inputs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Inputs</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Isothermal conditions between 0 & 40 C</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Isothermal conditions between 0 & 40 C</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<center> 4&deg;C shows negligible activity while 37&deg;C shows highest fluorescence </center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<center<ins class="diffchange diffchange-inline">> '''<font color=green</ins>>4&deg;C shows negligible activity while 37&deg;C shows highest fluorescence </center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Dynamic conditions eg. steps & ramps</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Dynamic conditions eg. steps & ramps</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<center>The system responded to stepping of temperature up and down</center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<center<ins class="diffchange diffchange-inline">>'''<font color=green</ins>>The system responded to stepping of temperature up and down<ins class="diffchange diffchange-inline"></font>''' </ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<center>Future work- DSred Express can be substituted for GFPmut3b</center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<center<ins class="diffchange diffchange-inline">>'''<font color=red</ins>>Future work- DSred Express can be substituted for GFPmut3b<ins class="diffchange diffchange-inline"></font>''' </ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<center>No activation energy of system was calculated to be 1.5kJ/mol</center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<center<ins class="diffchange diffchange-inline">>'''<font color=red</ins>>No activation energy of system was calculated to be 1.5kJ/mol<ins class="diffchange diffchange-inline"></font>''' </ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Health Regulations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Health Regulations</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must not be living replicating bacteria</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must not be living replicating bacteria</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<center>Yes through use of cell free chassis</center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<center<ins class="diffchange diffchange-inline">>'''<font color=green</ins>>Yes through use of cell free chassis<ins class="diffchange diffchange-inline"></font>''' </ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Response Time</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Response Time</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have a response time under 1 hour</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have a response time under 1 hour</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<center>Yes system increases significantly after 1 hour</center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<center<ins class="diffchange diffchange-inline">>'''<font color=green</ins>>Yes system increases significantly after 1 hour<ins class="diffchange diffchange-inline"></font>''' </ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Lifespan</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Lifespan</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must have a shelf life of 7 days</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must have a shelf life of 7 days</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<center>Not Determined- Packaging method that prevents evaporation</center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<center<ins class="diffchange diffchange-inline">>'''<font color=red</ins>>Not Determined- Packaging method that prevents evaporation<ins class="diffchange diffchange-inline"></font>''' </ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td></tr>
</table>Peixuanhttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=44582&oldid=prevPeixuan: /* Cell by Date: Conclusion */2007-10-26T20:32:50Z<p><span class="autocomment">Cell by Date: Conclusion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:32, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 34:</td>
<td colspan="2" class="diff-lineno">Line 34:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Dynamic conditions eg. steps & ramps</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Dynamic conditions eg. steps & ramps</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<center><del class="diffchange diffchange-inline">hmm</del></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<center><ins class="diffchange diffchange-inline">The system responded to stepping of temperature up and down</ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Outputs</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<center><del class="diffchange diffchange-inline">No we were unable to use a visual reporter</del></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<center><ins class="diffchange diffchange-inline">Future work- DSred Express can be substituted for GFPmut3b</ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Activation Energy</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 50:</td>
<td colspan="2" class="diff-lineno">Line 50:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Response Time</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Response Time</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have a response time under 1 hour</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System needs to have a response time under 1 hour</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<center><del class="diffchange diffchange-inline">hmm tbd</del></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<center><ins class="diffchange diffchange-inline">Yes system increases significantly after 1 hour</ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Lifespan</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Lifespan</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must have a shelf life of 7 days</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>System must have a shelf life of 7 days</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>||<center><del class="diffchange diffchange-inline">No with current packaging methods evaporation meant </del>that <del class="diffchange diffchange-inline">our system 'died' after 2 days</del></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>||<center><ins class="diffchange diffchange-inline">Not Determined- Packaging method </ins>that <ins class="diffchange diffchange-inline">prevents evaporation</ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br clear="all"></div></td></tr>
</table>Peixuanhttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=44463&oldid=prevPeixuan: /* Cell by Date: Conclusion */2007-10-26T20:18:01Z<p><span class="autocomment">Cell by Date: Conclusion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:18, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 29:</td>
<td colspan="2" class="diff-lineno">Line 29:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Inputs</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|style="background:#ffffcc"|Inputs</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Isothermal conditions between 0 & 40 C</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<center>Isothermal conditions between 0 & 40 C</center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<center> <del class="diffchange diffchange-inline">No </del></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<center> <ins class="diffchange diffchange-inline">4&deg;C shows negligible activity while 37&deg;C shows highest fluorescence </ins></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
</table>Peixuanhttp://2007.igem.org/wiki/index.php?title=Imperial/Cell_by_Date/Conclusion&diff=43493&oldid=prevAnthony L at 18:23, 26 October 20072007-10-26T18:23:42Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:23, 26 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 19:</td>
<td colspan="2" class="diff-lineno">Line 19:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__NOTOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__NOTOC__</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Cell by Date: Conclusion =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Cell by Date: Conclusion =</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{|border="1" width="80%" align="center"</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|width="20%" style="background:#ffffcc"|<center>'''Property'''</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|width="--"|<center>'''Value'''</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>'''Achieved ?'''</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|style="background:#ffffcc"|Inputs</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>Isothermal conditions between 0 & 40 C</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center> No </center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|style="background:#ffffcc"|</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>Dynamic conditions eg. steps & ramps</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">||<center>hmm</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|style="background:#ffffcc"|Outputs</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>No we were unable to use a visual reporter</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|style="background:#ffffcc"|Activation Energy</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">||<center>No activation energy of system was calculated to be 1.5kJ/mol</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|style="background:#ffffcc"|Health Regulations</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>System must not be living replicating bacteria</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>Yes through use of cell free chassis</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|style="background:#ffffcc"|Response Time</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>System needs to have a response time under 1 hour</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">||<center>hmm tbd</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|style="background:#ffffcc"|Lifespan</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|<center>System must have a shelf life of 7 days</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">||<center>No with current packaging methods evaporation meant that our system 'died' after 2 days</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br clear="all"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Conclusion still to do</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cell By Date is a great example of how synthetic biology can be integrated into our daily lives. When fully developed it can mimic the function of inducstrial level devices in the food industry at a fraction of the cost. Being a reporter of a break in the cold chain, CBD, can inform the end consumer whether the product he is buying has been exposed to abnormal storage conditions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cell By Date is a great example of how synthetic biology can be integrated into our daily lives. When fully developed it can mimic the function of inducstrial level devices in the food industry at a fraction of the cost. Being a reporter of a break in the cold chain, CBD, can inform the end consumer whether the product he is buying has been exposed to abnormal storage conditions.</div></td></tr>
</table>Anthony L