Revision as of 01:41, 27 October 2007

Cell by Date: Implementation

DNA Construct Implementation

Construct Design

Concept 1

Concept 2

Stage 1: Availability

Found as BBa_I13522 in Registry

Found as BBa_E7104 in Registry.

Stage 2: Cloning / Amplification

BBa_I13522 - Lane R2 (937bp + 2079bp vector)

BBa_E7104 - Lane 11 (Missing 826bp)

Stage 3: Ligation

Not Required

Since BBa_E7104 was not functional, we made our own promoter and and ligated it to BBa_I13504

+

=

Stage 4: Purification of Activator Proteins

Not Required

Stage 5: Quality Control

Stage 6: Final Implementation Status

Ready for Testing

Chassis Construct Implementation
Chassis Design
Stage 1: Availability	Possibility of making Homemade S30 Cell Extract.	Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation	Preparation: Done (Protocol)	Preparation: Done (Protocol)
Stage 3: Quality Control	Testing in vitro: Failed (Protocol and Results)	Testing in vitro: Passed (Protocol and Results)
Stage 4: Final Implementation Status	Aborted. Expression capacity of chassis was low.	Ready for Testing

<< Modelling | Implementation | Testing/Validation >>

@@ Line 86: / Line 86: @@
 |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
 |width="40%"|
-* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
+* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
 |width="40%"|
 * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.2 Results])