Imperial/Infector Detector/F2620 Comparison
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- | + | |<br>The basis for comparison is to normalise the ''in vitro'' chassis on the number of plasmids to give a platform for comparison: | |
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*''In Vitro'' - 4µg of DNA was added which for [http://partsregistry.org/Part:BBa_T9002 '''pTet-LuxR-pLux-GFPmut3b'''] is 904823007 plasmids | *''In Vitro'' - 4µg of DNA was added which for [http://partsregistry.org/Part:BBa_T9002 '''pTet-LuxR-pLux-GFPmut3b'''] is 904823007 plasmids | ||
*''In Vivo'' - Each cell the plasmids number was estimated at 30 per cell | *''In Vivo'' - Each cell the plasmids number was estimated at 30 per cell | ||
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#'''Rate of GFP synthesis''' of 100nM | #'''Rate of GFP synthesis''' of 100nM | ||
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Revision as of 19:39, 26 October 2007
Summary of Comparison
We compared our in vitro characterisation to the characterisation of F2620 in vivo with the aim to highlight some of the differences between the chassis and investigate how the constructs characteristics may change between them. The F2620 is an ideal construct to compare for comparison because of its detailed characterisation in vivo. The construct is the same as the construct 1 that was used for infecter detector, pTet-LuxR-pLux-GFPmut3b. The key results the comparison were;
- The creation of a new unit to allow comparison between in vitro and in vivo chassis.
- The constructs response appears to be largely independent of the chassis used.
Both of these are important findings and highlight the potential that new chassis offer to synthetic biology.
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