From 2007.igem.org

Revision as of 01:47, 24 October 2007 (view source) Alexander.wong (Talk | contribs) ← Older edit		Revision as of 10:52, 24 October 2007 (view source) Alexander.wong (Talk | contribs) Newer edit →
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	= Infector Detector: Implementation =		= Infector Detector: Implementation =
-	== DNA Construct Status ==
-	{|border=1 width=90% align="center"
-	|-
-	|width="40%" style="background-color:#eeeeff"| <center>'''Final Construct'''</center>
-	|width="--"  style="background-color:#eeeeff"| <center>'''Availability Status'''</center>
-	|-
-	|width="40%"|<center>[[Image:Imperial ID Design ID1.png]]</center>
-	|width="--"|This part can be found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in the registry
-	|-
-	|width="40%"|<center>[[Image:Imperial_ID_Design_ID2.png]]</center>
-	|width="--"|This part can be found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in the registry.
-	|}
-	<br clear="all">
-		+	{|border=1 width=100% align="center" cellpadding="10px"
-	=== Ligation Strategy ===	+
-	{|border=1 width=90% align="center"	+
	|-		|-
-	|width="40%" style="background-color:#eeeeff"| <center>'''DNA Construct'''</center>	+	|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center>
-	|width="--" style="background-color:#eeeeff"| <center>'''Ligation Strategy'''</center>	+	|- align="center"
-	|-	+	|wifth="20%" style="background-color:#ddeeff"| '''Construct Design'''
-	|width="40%"|<center>[[Image:Imperial ID Design ID1.png]]</center>	+	|width="40%"| [[Image:Imperial ID Design ID1.png]]
-	|width="--"| <center>Not Required</center>	+	|width="40%"| [[Image:Imperial_ID_Design_ID2.png]]
-	|-	+	|- align="center"
-	|width="40%"|<center>[[Image:Imperial_ID_Design_ID2.png]]</center>	+	|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
-	|width="--"| <center>Not Required</center>	+	|width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry.
		+	|width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry.
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
		+	|width="40%"| (Insert Gel Image)
		+	|width="40%"| (Insert Gel Image)
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
		+	|width="40%"| Not Required
		+	|width="40%"| Not Required
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
		+	|width="40%"| Not Required
		+	|width="40%"| LuxR protein (Protocols & Results)
		+	|-
		+	|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
		+	|width="40%"|
		+	* '''Testing ''in vivo''''': Done (Protocols & Results)
		+	* '''Sequencing of Construct''': Pending
		+	|width="40%"|
		+	* '''Testing ''in vivo''''': Done (Protocols & Results)
		+	* '''Sequencing of Construct''': Pending
		+	|- align="center"
		+	|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
		+	|width="40%"| Ready for Testing
		+	|width="40%"| Aborted. <br /> LuxR protein not functional.
	|}		|}
	<br clear="all">		<br clear="all">
-
-	=== Quality Control ===
-	[[Image:Imperial ID Design ID1.png]]
-
-	'''Quality Control:'''
-	{|border=1 width=90% align="center"
-	|-
-	|width="20%"|&nbsp;Testing ''in vivo''
-	|width="10%" align="center"|Done
-	|width="--"|Results can be found (Link to protocols and results)
-	|-
-	|&nbsp;Testing ''in vitro''
-	|align="center"|Done
-	|Results can be found at (Link to protocols and results)
-	|-
-
-	|&nbsp;Sequencing
-	|align="center"|Pending
-	|''Sequence results not obtained yet''
-	|}
-	<br clear="all">
-
-
-	[[Image:Imperial ID Design ID2.png]]<br>
-	'''Quality Control:'''
-	{|border=1 width=90% align="center"
-	|-
-	|width="20%"|&nbsp;Testing ''in vivo''
-	|width="10%" align="center"|Done
-	|width="--"|Results can be found (Link to protocols and results)
-	|-
-	|&nbsp;Testing ''in vitro''
-	|align="center"|Done
-	|Results can be found (Link to protocols and results)
-	|-
-
-	|&nbsp;Sequencing
-	|align="center"|Pending
-	|''Sequence results not obtained yet''
-	|}
-	<br clear="all">
	== Chassis Status ==		== Chassis Status ==

---

Revision as of 10:52, 24 October 2007

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

---

Infector Detector: Implementation

DNA Construct Implementation
Construct Design
Stage 1: Availability	Found as BBa_T9002 in Registry.	Found as BBa_J37032 in Registry.
Stage 2: Cloning / Amplification	(Insert Gel Image)	(Insert Gel Image)
Stage 3: Ligation	Not Required	Not Required
Stage 4: Purification of Activator Proteins	Not Required	LuxR protein (Protocols & Results)
Stage 5: Quality Control	Testing in vivo: Done (Protocols & Results) Sequencing of Construct: Pending	Testing in vivo: Done (Protocols & Results) Sequencing of Construct: Pending
Stage 6: Final Implementation Status	Ready for Testing	Aborted. LuxR protein not functional.

Chassis Status

Chassis	Availability Status
Promega E. coli S30 Cell Extract	Purchased

(Link to Commercial Preparation Protocols)

<< Modelling | Implementation | Testing/Validation >>