Revision as of 11:46, 24 October 2007

Infector Detector: Implementation

DNA Construct Implementation
Construct Design
Stage 1: Availability	Found as BBa_T9002 in Registry.	Found as BBa_J37032 in Registry.
Stage 2: Cloning / Amplification	BBa_T9002 - Lane 18 (1945bp)	BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation	Not Required	Not Required
Stage 4: Purification of Activator Proteins	Not Required	LuxR protein (Protocol and Results)
Stage 5: Quality Control	Testing in vivo: Done (Protocol and Results) Sequencing of Construct: Pending	Testing in vitro: Done (Protocol and Results) Sequencing of Construct: Pending
Stage 6: Final Implementation Status	Ready for Testing	Aborted. LuxR protein not functional.

Chassis	Availability Status
Promega E. coli S30 Cell Extract	Purchased

(Link to Commercial Preparation Protocols)

<< Modelling | Implementation | Testing/Validation >>

@@ Line 36: / Line 36: @@
 |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
 |width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]]
-|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + vector)]]
+|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]]
 |- align="center"
 |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''