Imperial/Infector Detector/Implementation
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|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center> | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center> | ||
|- align="center" | |- align="center" | ||
- | |wifth="20%" style="background-color:#ddeeff"| '''Construct Design''' | + | |wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' |
- | |width="40%" align="left" | '''Concept 1''' <br />[[Image:Imperial ID Design ID1.png|center]] | + | |width="40%" align="left" | '''Concept 1''' <br />[[Image:Imperial ID Design ID1.png|center|]] |
|width="40%" align="left" | '''Concept 2''' <br />[[Image:Imperial_ID_Design_ID2.png|center]] | |width="40%" align="left" | '''Concept 2''' <br />[[Image:Imperial_ID_Design_ID2.png|center]] | ||
|- align="center" | |- align="center" | ||
- | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. | |width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. | ||
|width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. | |width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. | ||
|- align="center" | |- align="center" | ||
- | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]] | |width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]] | ||
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]] | |width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]] | ||
|- align="center" | |- align="center" | ||
- | |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| Not Required | |width="40%"| Not Required | ||
|width="40%"| Not Required | |width="40%"| Not Required | ||
|- align="center" | |- align="center" | ||
- | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] |
+ | |||
|width="40%"| Not Required | |width="40%"| Not Required | ||
|width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]) | |width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]) | ||
|- | |- | ||
- | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | + | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vivo''''': | + | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]) |
* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]) |
* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
|- align="center" | |- align="center" | ||
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|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]] | |colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]] | ||
|- align="center" | |- align="center" | ||
- | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| Possibility of making Homemade S30 Cell Extract. | |width="40%"| Possibility of making Homemade S30 Cell Extract. | ||
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. | |width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. | ||
|- align="center" | |- align="center" | ||
- | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' | + | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]) | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]) | ||
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | ||
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' | ||
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Passed (Protocol and Results) |
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]) |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' |
Revision as of 14:27, 24 October 2007
Infector Detector: Implementation
| ||
Construct Design | Concept 1 | Concept 2 |
Stage 1: Availability | Found as BBa_T9002 in Registry. | Found as BBa_J37032 in Registry. |
Stage 2: Cloning / Amplification | ||
Stage 3: Ligation | Not Required | Not Required |
Stage 4: Purification of Activator Proteins | Not Required | LuxR protein (Protocol and Results) |
Stage 5: Quality Control | ||
Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
| ||
Chassis Design | ||
Stage 1: Availability | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from Promega. |
Stage 2: Preparation | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control |
| |
Stage 6: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |