Imperial/Infector Detector/Implementation
From 2007.igem.org
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|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | ||
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| - | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' | + | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] |
|width="40%"| | |width="40%"| | ||
* '''Testing ''in vitro''''': Passed (Protocol and Results) | * '''Testing ''in vitro''''': Passed (Protocol and Results) | ||
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* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]) | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]) | ||
|- align="center" | |- align="center" | ||
| - | |width="20%" style="background-color:#ddeeff"| '''Stage | + | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' |
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | |width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | ||
|width="40%" style="background-color:#b8efb0"| Ready for Testing | |width="40%" style="background-color:#b8efb0"| Ready for Testing | ||
Revision as of 14:28, 24 October 2007
Infector Detector: Implementation
| | ||
| Construct Design | Concept 1  | Concept 2  |
Stage 1: Availability 
| Found as BBa_T9002 in Registry. | Found as BBa_J37032 in Registry. |
| Stage 2: Cloning / Amplification
|  |  |
| Stage 3: Ligation
| Not Required | Not Required |
| Stage 4: Purification of Activator Proteins
| Not Required | LuxR protein (Protocol and Results) |
| Stage 5: Quality Control
| ||
| Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
| | ||
| Chassis Design | 
| |
| Stage 1: Availability
| Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from Promega. |
| Stage 2: Preparation
| Preparation: Done (Protocol) | Preparation: Done (Protocol) |
| Stage 3: Quality Control
|
| |
| Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |
