Imperial/Infector Detector/Implementation
From 2007.igem.org
(Difference between revisions)
(→Infector Detector: Implementation) |
|||
| Line 24: | Line 24: | ||
{|border=1 width=100% align="center" cellpadding="10px" | {|border=1 width=100% align="center" cellpadding="10px" | ||
|- | |- | ||
| - | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center> | + | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation''' </center> |
| + | |- | ||
| + | |colspan="3" style="background-color:#eeeeff"|[[Image:IC07_design_ID.png|center|500px]] | ||
|- align="center" | |- align="center" | ||
|wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' | |wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' | ||
| - | |width="40%" align="left" | | + | |width="40%" align="left" | [[Image:BB_c1.png|center|300px]] |
| - | |width="40%" align="left" | | + | |width="40%" align="left" | [[Image:BB_c2.png|center|300px]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
| - | |width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. | + | |width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. <br />[[Image:Imperial ID Design ID1.png|center|]] |
| - | |width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. | + | |width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. <br /> [[Image:Imperial_ID_Design_ID2.png|center]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
Revision as of 09:17, 25 October 2007
Infector Detector: Implementation
| | ||
 | ||
| Construct Design |  |  |
Stage 1: Availability 
| Found as BBa_T9002 in Registry.  | Found as BBa_J37032 in Registry.  |
| Stage 2: Cloning / Amplification
|  |  |
| Stage 3: Ligation
| Not Required | Not Required |
| Stage 4: Purification of Activator Proteins
| Not Required | LuxR protein (Protocol and Results) |
| Stage 5: Quality Control
| ||
| Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
| | ||
| Chassis Design | 
| |
| Stage 1: Availability
| Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from Promega. |
| Stage 2: Preparation
| Preparation: Done (Protocol) | Preparation: Done (Protocol) |
| Stage 3: Quality Control
|
| |
| Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |
