Imperial/Infector Detector/Implementation
From 2007.igem.org
(Difference between revisions)
| Line 46: | Line 46: | ||
|width="40%"| Not Required | |width="40%"| Not Required | ||
| - | |width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results]) | + | |width="40%"| [[Image:Purification_of_LuxR.PNG|center|300px]] LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results]) |
|- | |- | ||
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
| Line 53: | Line 53: | ||
* '''Sequencing of Construct''': Completed | * '''Sequencing of Construct''': Completed | ||
|width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br /> | |width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br /> | ||
| - | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results]) |
* '''Sequencing of Construct''': Completed | * '''Sequencing of Construct''': Completed | ||
|- align="center" | |- align="center" | ||
Revision as of 01:36, 26 October 2007
Infector Detector: Implementation
| | ||
| Construct Design |  |  |
Stage 1: Availability 
| Found as BBa_T9002 in Registry.  | Found as BBa_J37032 in Registry.  |
| Stage 2: Cloning / Amplification
|  |  |
| Stage 3: Ligation
| Not Required | Not Required |
| Stage 4: Purification of Activator Proteins
| Not Required |  |
| Stage 5: Quality Control
|  |  |
| Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
| | ||
| Chassis Design | 
| |
| Stage 1: Availability
| Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from Promega. |
| Stage 2: Preparation
| Preparation: Done (Protocol) | Preparation: Done (Protocol) |
| Stage 3: Quality Control
|
| |
| Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |
