Imperial/Infector Detector/Implementation

From 2007.igem.org

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     <li><a class="current" href="https://2007.igem.org/Imperial/Infector_Detector/Implementation" title=""><span>Implementation</span></a></li>
     <li><a class="current" href="https://2007.igem.org/Imperial/Infector_Detector/Implementation" title=""><span>Implementation</span></a></li>
     <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Testing" title=""><span>Testing</span></a></li>
     <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Testing" title=""><span>Testing</span></a></li>
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    <li><a href="https://2007.igem.org/Imperial/Infector_Detector/F2620 Comparison" title=""><span>F2620</span></a></li>
     <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Conclusion" title=""><span>Conclusion</span></a></li>
     <li><a href="https://2007.igem.org/Imperial/Infector_Detector/Conclusion" title=""><span>Conclusion</span></a></li>
   </ul>
   </ul>
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{|border=1 width=100% align="center" cellpadding="10px"
{|border=1 width=100% align="center" cellpadding="10px"
|-
|-
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|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center>
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|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation''' </center>
|- align="center"
|- align="center"
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|wifth="20%" style="background-color:#ddeeff"| '''Construct Design'''
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|wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design'''
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|width="40%" align="left" | '''Concept 1''' <br />[[Image:Imperial ID Design ID1.png|center]]
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|width="40%" align="left" | [[Image:IC07_design_ID.png|center|370px]]
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|width="40%" align="left" | '''Concept 2''' <br />[[Image:Imperial_ID_Design_ID2.png|center]]
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|width="40%" align="left" | [[Image:IC07_design_ID-con2.png|center|370px]]
|- align="center"
|- align="center"
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|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
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|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
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|width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry.
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|width="40%"| Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. <br />[[Image:Imperial ID Design ID1.png|center|]]
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|width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry.
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|width="40%"| Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. <br /> [[Image:Imperial_ID_Design_ID2.png|center]]
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
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|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]]
|width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]]
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]]
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]]
|- align="center"
|- align="center"
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|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
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|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Not Required
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
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|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]]
 +
 
|width="40%"| Not Required
|width="40%"| Not Required
-
|width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results])
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|width="40%"| [[Image:Purification_of_LuxR.PNG|center|thumb|200px|Purification of LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results])]]
|-  
|-  
-
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]]
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|width="40%"|  
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|width="40%"| [[Image:IC_2007_Plux_in_vivo.PNG|center|350px]]<br />
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* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results])
+
* '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.1 Results])
-
* '''Sequencing of Construct''': Pending
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* '''Sequencing of Construct''': Partially Sequenced
-
|width="40%"|  
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|width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br />
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* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results])  
+
* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results])  
-
* '''Sequencing of Construct''': Pending
+
* '''Sequencing of Construct''': Completed
|- align="center"
|- align="center"
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
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|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
|- align="center"
|- align="center"
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|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Possibility of making Homemade S30 Cell Extract.
|width="40%"| Possibility of making Homemade S30 Cell Extract.
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
|- align="center"
|- align="center"
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|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation'''
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|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
|-  
|-  
-
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control'''
+
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vitro''''': Done (Protocol and Results)
+
* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results])  
+
* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.2 Results])  
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status'''
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
|width="40%" style="background-color:#b8efb0"| Ready for Testing
|width="40%" style="background-color:#b8efb0"| Ready for Testing

Latest revision as of 02:33, 27 October 2007




Infector Detector: Implementation

DNA Construct Implementation
Construct Design
IC07 design ID.png
IC07 design ID-con2.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png
Found as BBa_T9002 in Registry.
Imperial ID Design ID1.png
Found as BBa_J37032 in Registry.
Imperial ID Design ID2.png
Stage 2: Cloning / Amplification

Icegems-Devcycle-downarrow.png
BBa_T9002 - Lane 18 (1945bp)
BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation

Icegems-Devcycle-downarrow.png
Not Required Not Required
Stage 4: Purification of Activator Proteins

Icegems-Devcycle-downarrow.png
Not Required
Purification of LuxR protein (Protocol and Results)
Stage 5: Quality Control

Icegems-Devcycle-downarrow.png
IC 2007 Plux in vivo.PNG

  • Testing in vivo: Passed (Protocol and Results)
  • Sequencing of Construct: Partially Sequenced
IC 2007 LuxR testing 1.PNG

  • Testing in vitro: Failed (Protocol and Results)
  • Sequencing of Construct: Completed
Stage 6: Final Implementation Status Ready for Testing Aborted.
LuxR protein not functional.



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png
Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation

Icegems-Devcycle-downarrow.png
Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control

Icegems-Devcycle-downarrow.png
Stage 4: Final Implementation Status Aborted.
Expression capacity of chassis was low.
Ready for Testing



<< Modelling | Implementation | Testing/Validation >>