Imperial/Infector Detector/Implementation
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|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation''' </center> | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation''' </center> | ||
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|wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' | |wifth="20%" style="background-color:#ddeeff" border="0" | '''Construct Design''' | ||
- | |width="40%" align="left" | [[Image: | + | |width="40%" align="left" | [[Image:IC07_design_ID.png|center|370px]] |
- | |width="40%" align="left" | [[Image: | + | |width="40%" align="left" | [[Image:IC07_design_ID-con2.png|center|370px]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
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|width="40%"| Not Required | |width="40%"| Not Required | ||
- | |width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [ | + | |width="40%"| [[Image:Purification_of_LuxR.PNG|center|thumb|200px|Purification of LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results])]] |
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
- | |width="40%"| | + | |width="40%"| [[Image:IC_2007_Plux_in_vivo.PNG|center|350px]]<br /> |
- | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [ | + | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.1 Results]) |
- | * '''Sequencing of Construct''': | + | * '''Sequencing of Construct''': Partially Sequenced |
- | |width="40%"| | + | |width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br /> |
- | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results]) |
- | * '''Sequencing of Construct''': | + | * '''Sequencing of Construct''': Completed |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | ||
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]]) |
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [ | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.2 Results]) |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' |
Latest revision as of 02:33, 27 October 2007
Infector Detector: Implementation
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Construct Design | ||
Stage 1: Availability | Found as BBa_T9002 in Registry. | Found as BBa_J37032 in Registry. |
Stage 2: Cloning / Amplification | ||
Stage 3: Ligation | Not Required | Not Required |
Stage 4: Purification of Activator Proteins | Not Required | |
Stage 5: Quality Control | | |
Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
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Chassis Design | ||
Stage 1: Availability | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from Promega. |
Stage 2: Preparation | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control | ||
Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |