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• Infector Detector
  • Introduction
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  • Implementation
  • Testing/Validation
  • F2620 Comparison
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• Cell by Date
  • Introduction
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  • Modelling
  • Implementation
  • Testing/Validation
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Infector Detector: Implementation

DNA Construct Implementation
Construct Design
Stage 1: Availability	Found as BBa_T9002 in Registry.	Found as BBa_J37032 in Registry.
Stage 2: Cloning / Amplification	BBa_T9002 - Lane 18 (1945bp)	BBa_J37032 - Lane R25 (938bp + vector)
Stage 3: Ligation	Not Required	Not Required
Stage 4: Purification of Activator Proteins	Not Required	LuxR protein (Protocol and Results)
Stage 5: Quality Control	Testing in vivo: Done (Protocol and Results) Sequencing of Construct: Pending	Testing in vitro: Done (Protocol and Results) Sequencing of Construct: Pending
Stage 6: Final Implementation Status	Ready for Testing	Aborted. LuxR protein not functional.

Chassis Status

Chassis	Availability Status
Promega E. coli S30 Cell Extract	Purchased

(Link to Commercial Preparation Protocols)

<< Modelling | Implementation | Testing/Validation >>