Imperial/Infector Detector/Implementation

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Infector Detector: Implementation

DNA Construct Implementation
Construct Design Concept 1
Imperial ID Design ID1.png
Concept 2
Imperial ID Design ID2.png
Stage 1: Availability Found as BBa_T9002 in Registry. Found as BBa_J37032 in Registry.
Stage 2: Cloning / Amplification
BBa_T9002 - Lane 18 (1945bp)
BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation Not Required Not Required
Stage 4: Purification of Activator Proteins Not Required LuxR protein (Protocol and Results)
Stage 5: Quality Control
  • Testing in vivo: Done (Protocol and Results)
  • Sequencing of Construct: Pending
  • Testing in vitro: Done (Protocol and Results)
  • Sequencing of Construct: Pending
Stage 6: Final Implementation Status Ready for Testing Aborted.
LuxR protein not functional.



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control
  • Testing in vitro: Done (Protocol and Results)
Stage 6: Final Implementation Status Aborted.
Expression capacity of chassis was low.
Ready for Testing



<< Modelling | Implementation | Testing/Validation >>