Imperial/Infector Detector/Specification

From 2007.igem.org

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== Specifications in detail ==
== Specifications in detail ==
[[Image:IC07 spec sysresponse.png|thumb|400px|left|'''Desired response''' of Infector Detector. Note that the response is not necessarily linear.]]
[[Image:IC07 spec sysresponse.png|thumb|400px|left|'''Desired response''' of Infector Detector. Note that the response is not necessarily linear.]]
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* '''Input: AHL 5-50nM'''  
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* '''Input: AHL 5-50nM'''
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It is known that, in Pseudomonas ''aeruginosa'' biofilms, the concentration of AHL is at least 5nM. ([[#References|1]]) Therefore, if the system can successfully report the presence of this concentration, it should be able to detect such biofilms.
* '''Output: Visible fluorescent protein'''
* '''Output: Visible fluorescent protein'''
* '''Response Time: < 3 hours'''
* '''Response Time: < 3 hours'''
* '''Operating conditions: Temperatures from 20&deg; to 30&deg;C'''
* '''Operating conditions: Temperatures from 20&deg; to 30&deg;C'''
* '''Health & safety: Non-living, non-infectious'''
* '''Health & safety: Non-living, non-infectious'''
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* '''Packaging and shelf-life'''
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* '''Packaging and shelf-life of 7 days'''
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== References ==
== References ==

Revision as of 22:04, 22 October 2007


Infector Detector: Specifications

The system must be able to detect the presence of biofilms on urinary catheters by detection of AHL, at a minimum concentration of 5nM, and report with a visual signal within 3 hours. It must work within a temperature range of 20°-30°C, be portable and easy to use, have a shelf life of at least seven days, and must not be harmful or infectious.


Property
Value
Inputs
System must be sensitive to AHL concentration between 5-50nM
Outputs
System must give a visual signal if bacteria is present
Response Time
System needs to have a response time under 3 hour
Operating Conditions
System must operate within temperature 20-30°C
Health & Safety
System Must not be living replicating bacteria, and in any way harmful or infectious.
Lifespan
System must have a shelf life of 7 days
Packaging
System must be portable and convenient to use


Specifications in detail

Desired response of Infector Detector. Note that the response is not necessarily linear.
  • Input: AHL 5-50nM

It is known that, in Pseudomonas aeruginosa biofilms, the concentration of AHL is at least 5nM. (1) Therefore, if the system can successfully report the presence of this concentration, it should be able to detect such biofilms.

  • Output: Visible fluorescent protein
  • Response Time: < 3 hours
  • Operating conditions: Temperatures from 20° to 30°C
  • Health & safety: Non-living, non-infectious
  • Packaging and shelf-life of 7 days


References

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