Imperial/Wet Lab/Lab Notebook/2007-08-01

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__NOTOC__
= 1 August 2007 =
= 1 August 2007 =
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===Preparation of Stock Solutions===
 +
*1x 1 litre of 10x TBE
 +
*20x 100 ml of LB media in conical flasks
 +
 +
===Preparation of Competent Cells===
 +
#Poured LB agar plates
 +
#*Nalixidic acid (15 μg/ml)
 +
#*Tetracyclin (20 μg/ml)
 +
#Streaked E.coli cells on agar plates
 +
#*DH5Alpha
 +
#*XL1Blue
 +
#Incubated plates at 37°C overnight
 +
 +
===Preparation of Equipment===
 +
*1x 37°C shaking incubator
 +
*1x 4°C fridge
 +
*1x -20°C freezer
 +
*2x Weighing scales
 +
*3x Magnetic stirrer
 +
*3x Microcentrifuge
 +
*3x Vortex machines
 +
*2x UV Spectrometer
 +
*5x Gilson pipettes (P1000, P200, P20, P10, P2)
 +
*20 boxes x Tips (yellow and blue)
 +
*5 boxes x Small tips
 +
*Eppendorf tubes
 +
*Centrifuge tubes
 +
*50x 5 ml pipettes
 +
*50x 10 ml pipettes
 +
*50x 25 ml pipettes
 +
*5x Pipette fillers
 +
*37°C water bath
 +
*15°C water bath
 +
*Driblock
 +
*10 packets x Innoculation loops
 +
*3x Bunsen burners
 +
*3 boxes Matches
 +
*3x Agarose gel equipments
 +
*Microwave oven
 +
*Transilluminator
 +
*Camera
 +
*3x Scapel (for cutting agarose gel)
 +
*1 litre bottles
 +
*Beakers
 +
*Conical flasks
 +
*Volumetric flasks
 +
*Measuring cylinders
 +
*Gloves
 +
*Goggles
 +
*Markers
 +
*Sticker labels
 +
*Paper towels
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Latest revision as of 10:49, 9 October 2007


1 August 2007

Preparation of Stock Solutions

  • 1x 1 litre of 10x TBE
  • 20x 100 ml of LB media in conical flasks

Preparation of Competent Cells

  1. Poured LB agar plates
    • Nalixidic acid (15 μg/ml)
    • Tetracyclin (20 μg/ml)
  2. Streaked E.coli cells on agar plates
    • DH5Alpha
    • XL1Blue
  3. Incubated plates at 37°C overnight

Preparation of Equipment

  • 1x 37°C shaking incubator
  • 1x 4°C fridge
  • 1x -20°C freezer
  • 2x Weighing scales
  • 3x Magnetic stirrer
  • 3x Microcentrifuge
  • 3x Vortex machines
  • 2x UV Spectrometer
  • 5x Gilson pipettes (P1000, P200, P20, P10, P2)
  • 20 boxes x Tips (yellow and blue)
  • 5 boxes x Small tips
  • Eppendorf tubes
  • Centrifuge tubes
  • 50x 5 ml pipettes
  • 50x 10 ml pipettes
  • 50x 25 ml pipettes
  • 5x Pipette fillers
  • 37°C water bath
  • 15°C water bath
  • Driblock
  • 10 packets x Innoculation loops
  • 3x Bunsen burners
  • 3 boxes Matches
  • 3x Agarose gel equipments
  • Microwave oven
  • Transilluminator
  • Camera
  • 3x Scapel (for cutting agarose gel)
  • 1 litre bottles
  • Beakers
  • Conical flasks
  • Volumetric flasks
  • Measuring cylinders
  • Gloves
  • Goggles
  • Markers
  • Sticker labels
  • Paper towels