Imperial/Wet Lab/Lab Notebook/2007-08-08

From 2007.igem.org

(Difference between revisions)
(8 August 2007)
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= 8 August 2007 =
= 8 August 2007 =
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==Transformation of Biobricks==
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#15 biobricks were transformed into XL1-Blue
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#Cells were grown for 1 hour and plated
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#Plates were at 37°C overnight
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<font size=-2>
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*1. BBa_E0040 [GFP]
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*2. BBa_I13352 [ptet-GFP]
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*3. BBa_J5528 [pBad-GFP]
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*4. BBa_J24677 [ptet-LuxR-plux-GFP]
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*5. BBa_R0040 [ptet]
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*6. BBa_I0500 [pBad]
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*7. BBa_F2620 [ptet-LuxR-plux]
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*8. BBa_F1610 [luxI]
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*9. BBa_R0010 [lacI]
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*10.BBa_E7010 [RFP]
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*11.BBa_E7104 [T7 GFP]
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*12.BBa_S03550 [araC]
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*13.BBa_B0015 [Terminator]
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*14.BBa_R0051 [pCI]
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*15.BBa_S07037 [pCI-GFP]
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</font>
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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electroporation|Electroporation]] in the general protocols page
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*Plate 3 had no colony (possibly due to large plasmid size - difficult to transform)
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==Preparation of Competent Cells==
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#BL21(DE3)-Gold was made electrocompetent
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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Preparation_of_Competent_Cells|Preparation of Competent Cells]] in the general protocols page
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Revision as of 13:36, 10 October 2007

8 August 2007

Transformation of Biobricks

  1. 15 biobricks were transformed into XL1-Blue
  2. Cells were grown for 1 hour and plated
  3. Plates were at 37°C overnight

  • 1. BBa_E0040 [GFP]
  • 2. BBa_I13352 [ptet-GFP]
  • 3. BBa_J5528 [pBad-GFP]
  • 4. BBa_J24677 [ptet-LuxR-plux-GFP]
  • 5. BBa_R0040 [ptet]
  • 6. BBa_I0500 [pBad]
  • 7. BBa_F2620 [ptet-LuxR-plux]
  • 8. BBa_F1610 [luxI]
  • 9. BBa_R0010 [lacI]
  • 10.BBa_E7010 [RFP]
  • 11.BBa_E7104 [T7 GFP]
  • 12.BBa_S03550 [araC]
  • 13.BBa_B0015 [Terminator]
  • 14.BBa_R0051 [pCI]
  • 15.BBa_S07037 [pCI-GFP]

Protocols can be found at Electroporation in the general protocols page

  • Plate 3 had no colony (possibly due to large plasmid size - difficult to transform)

Preparation of Competent Cells

  1. BL21(DE3)-Gold was made electrocompetent

Protocols can be found at Preparation of Competent Cells in the general protocols page