Imperial/Wet Lab/Lab Notebook/2007-08-10

From 2007.igem.org

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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page
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Revision as of 14:37, 10 October 2007

Contents

10 August 2007

Preparation of Stock Solutions

  • 1x 500 ml of 2M Tris-acetate pH 8.2
  • 1x 500 ml of Buffer A for preparation of S30/S12 cell extract
  • 1x 500 ml of Buffer B for preparation of S30/S12 cell extract
  • 1x 50 ml of 2mM amino acids

Miniprep of Biobricks

  1. Minipreped 2x 14 Biobricks

Protocols can be found at Miniprep in the general protocols page

Agarose Gel Electrophoresis of Biobricks

  1. Checked 2x 14 Biobricks on 1% agarose gel

Protocols can be found at Electrophoresis in the general protocols page

IC07 Gel 1.JPG IC07 Gel 3.JPG IC07 Gel 2.JPG