Imperial/Wet Lab/Lab Notebook/2007-08-23

From 2007.igem.org

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(23 August 2007)
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#*Phosphoenolpyruvate to be added tomorrow
#*Phosphoenolpyruvate to be added tomorrow
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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30/S12 Cell Extract|S30/S12 Cell Extract]] in the general protocols page
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Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30.2FS12_Cell_Extract#S30/S12 Cell Extract| S30/S12 Cell Extract] in the general protocols page
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==Vesicles Formation with GFP==
==Vesicles Formation with GFP==

Revision as of 12:51, 26 October 2007

23 August 2007

Preparation of Reaction Buffer for S30 Cell Extract

  1. Calculated and weighed out required amounts of reagents
  2. Left mixture on the stirrer in the cold room overnight
    • Phosphoenolpyruvate to be added tomorrow

Protocols can be found at Cell Extract| S30/S12 Cell Extract in the general protocols page

Vesicles Formation with GFP

Preparations

The desiccation and suspension stages of the vesicle preparation protocol were followed.

  • One 100ml beaker was prepared with 0.5mg of powdered POPC in 50ml of dodecane.
  • The suspension was sonicated for 30mins and left overnight in a 27°C incubator.