Imperial/Wet Lab/Lab Notebook/2007-09-02
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< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
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+ | ==Construction of pT7-GFP== | ||
+ | #5 colonies grew in the ligation plate | ||
+ | #All 5 colonies were picked and each was grown in 5 ml of LB + Kan | ||
+ | #The 5 cultures were incubated at 37°C overnight | ||
+ | |||
+ | ==Cloning of Biobricks (for Maxiprep)== | ||
+ | #Placed 200 ml of LB in a 500 ml conical flask | ||
+ | #Innoculated 2 ml of culture into LB media in flask | ||
+ | #Incubated overnight at 37 °C | ||
+ | |||
+ | <font size=-2> | ||
+ | *2. BBa_I13422 [ptet-GFP] | ||
+ | *18. BBa_T9002 [plux-GFP] | ||
+ | </font> | ||
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{{Template:IC07labnotebook}} | {{Template:IC07labnotebook}} |
Latest revision as of 14:06, 10 October 2007
2 September 2007
Construction of pT7-GFP
- 5 colonies grew in the ligation plate
- All 5 colonies were picked and each was grown in 5 ml of LB + Kan
- The 5 cultures were incubated at 37°C overnight
Cloning of Biobricks (for Maxiprep)
- Placed 200 ml of LB in a 500 ml conical flask
- Innoculated 2 ml of culture into LB media in flask
- Incubated overnight at 37 °C
- 2. BBa_I13422 [ptet-GFP]
- 18. BBa_T9002 [plux-GFP]