http://2007.igem.org/wiki/index.php?title=Imperial/Wet_Lab/Lab_Notebook/2007-09-24&feed=atom&action=historyImperial/Wet Lab/Lab Notebook/2007-09-24 - Revision history2024-03-29T06:55:01ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Imperial/Wet_Lab/Lab_Notebook/2007-09-24&diff=24562&oldid=prevLucasCY: /* 24 September 2007 */2007-10-10T14:12:37Z<p><span class="autocomment">24 September 2007</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 14:12, 10 October 2007</td>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Purification of LuxR==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*On Friday we harvested cells produciong LuxR and collected the inclusion bodies. Today we carried out refolding and ran an affinity column to seperate out the His-LuxR</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*At the end of the experiment we ended up with several fractions, when the elution profile was looked at there was only two peaks, one very slight one and one very step one for the last fraction</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*We will run a gel tomorrow to determine where the LuxR</ins></div></td></tr>
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</table>LucasCYhttp://2007.igem.org/wiki/index.php?title=Imperial/Wet_Lab/Lab_Notebook/2007-09-24&diff=24172&oldid=prevDirkvs at 10:43, 9 October 20072007-10-09T10:43:04Z<p></p>
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